Publication detail

Anodic formation and biomedical properties of hafnium-oxide nanofilms

FOHLEROVÁ, Z. MOZALEV, A.

Original Title

Anodic formation and biomedical properties of hafnium-oxide nanofilms

Type

journal article in Web of Science

Language

English

Original Abstract

Hafnium dioxide (HfO2) is attracting attention for bio-related applications due to its good cytocompatibility, high density, and resistance to corrosion and mechanical damage. Here we synthesize two types of hafnium-oxide thin films on substrates via self-organized electrochemical anodization: an array of hierarchically structured nanorods anchored to a thin oxide layer and a microscopically flat oxide film. The nanostructured film is composed of a unique mixture of HfO2, suboxide Hf2O3, and oxide-hydroxide compound HfO2 nH2O whereas the flat film is mainly HfO2. In vitro interaction of the two films with MG 63 osteoblast-like cells and gram-negative E coli bacteria is studied for the first time to assess the potential of the films for biomedical application. Both films reveal good cytocompatibility and affinity for proteins, represented by fibronectin and especially albumin, which is absorbed in nine times larger amount. The morphology and specific surface chemistry of the nanostructured film cause a two-fold enhanced antibacterial effect, better cell attachment, significantly improved proliferation of cells, five-fold rise in the cellular Young modulus, slightly stronger production of reactive oxygen species, and formation of cell clusters. Compared with the flat film, the nanostructured one features the weakening of AFM measured adhesion force at the cell surface interface, probably caused by partially lifting the nanorods from the substrate due to the strong contact with cells. The present findings deepen the understanding of biological processes at the living cell metal-oxide interface, underlying the role of surface chemistry and the impact of nanostructuring at the nanoscale.

Keywords

hafnia, nanostructures, bacteria, tissue cells, proteins

Authors

FOHLEROVÁ, Z.; MOZALEV, A.

Released

27. 2. 2019

Publisher

Royal Society of Chemistry

ISBN

2050-750X

Periodical

Journal of Materials Chemistry B

Year of study

7

Number

14

State

United Kingdom of Great Britain and Northern Ireland

Pages from

2300

Pages to

2310

Pages count

11

URL

https://pubs.rsc.org/en/content/articlelanding/2019/TB/C8TB03180K#!divAbstract

BibTex

@article{BUT155884,
  author="Zdenka {Fohlerová} and Alexander {Mozalev}",
  title="Anodic formation and biomedical properties of hafnium-oxide nanofilms",
  journal="Journal of Materials Chemistry B",
  year="2019",
  volume="7",
  number="14",
  pages="2300--2310",
  doi="10.1039/c8tb03180k",
  issn="2050-750X",
  url="https://pubs.rsc.org/en/content/articlelanding/2019/TB/C8TB03180K#!divAbstract"
}