Publication detail

Gold Nanoparticles as Labels for Immunochemical Analysis Using Laser Ablation Inductively Coupled Plasma Mass Spectrometry

TVRDOŇOVÁ, M. VLČNOVSKÁ, M. POMPEIANO VANÍČKOVÁ, L. KANICKÝ, V. ADAM, V. ASCHER, L. JAKUBOWSKI, N. VACULOVIČOVÁ, M. VACULOVIČ, T.

Original Title

Gold Nanoparticles as Labels for Immunochemical Analysis Using Laser Ablation Inductively Coupled Plasma Mass Spectrometry

Type

journal article in Web of Science

Language

English

Original Abstract

In this paper, we describe the labelling of antibodies by gold nanoparticles (AuNPs) with diameters of 10 and 60nm with detection by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Additionally, the AuNPs labelling strategy is compared with commercially available labelling reagents based on MeCAT (metal coded affinity tagging). Proof of principle experiments based on dot blot experiments were performed. The two labelling methods investigated were compared by sensitivity and limit of detection (LOD). The absolute LODs achieved were in the range of tens of picograms for AuNP labelling compared to a few hundred picograms by the MeCAT labelling.

Keywords

Dot blot; Antibody labelling; Gold nanoparticles; LA-ICP-MS; Immunochemistry

Authors

TVRDOŇOVÁ, M.; VLČNOVSKÁ, M.; POMPEIANO VANÍČKOVÁ, L.; KANICKÝ, V.; ADAM, V.; ASCHER, L.; JAKUBOWSKI, N.; VACULOVIČOVÁ, M.; VACULOVIČ, T.

Released

31. 1. 2019

ISBN

1618-2642

Periodical

Analytical and Bioanalytical Chemistry

Year of study

411

Number

3

State

Federal Republic of Germany

Pages from

559

Pages to

564

Pages count

6

BibTex

@article{BUT156134,
  author="Michaela {Tvrdoňová} and Marcela {Vlčnovská} and Lucie {Pompeiano Vaníčková} and Viktor {Kanický} and Vojtěch {Adam} and Lena {Ascher} and Norbert {Jakubowski} and Markéta {Vaculovičová} and Tomáš {Vaculovič}",
  title="Gold Nanoparticles as Labels for Immunochemical Analysis Using Laser Ablation Inductively Coupled Plasma Mass Spectrometry",
  journal="Analytical and Bioanalytical Chemistry",
  year="2019",
  volume="411",
  number="3",
  pages="559--564",
  doi="10.1007/s00216-018-1300-7",
  issn="1618-2642"
}