Publication detail

Utilization of antibody-nanoparticle conjugates as a tool for immunochemistry with ICP-MS detection

VLČNOVSKÁ, M. TVRDOŇOVÁ, M. VACULOVIČOVÁ, M. VACULOVIČ, T.

Original Title

Utilization of antibody-nanoparticle conjugates as a tool for immunochemistry with ICP-MS detection

Type

conference paper

Language

English

Original Abstract

Immunoanalytical techniques are key methods of application in clinical diagnostics, genomics, proteomics and other biochemical and molecular biology disciplines. Most often, they are based on the ability of labeled antibodies to bind specific antigens. It is possible to use a large variety of nanomaterials that are designed, synthesized and adapted to allow highly sensitive detection of advanced immunoassays. Detection can be a highly efficient analytical method of laser ablation followed by inductively coupled plasma mass spectrometry (LA-ICP-MS), which allows the detection of elemental tags suitably conjugated to antibodies. The aim of this work was to conjugate model anti-mouse antibody on a surface of 10nm and 60nm gold nanoparticles and choose the better one for conjugation experimentally by using dot-blot immunobinding assay followed by LA-ICP-MS. It has been experimentally proven that 10nm gold nanoparticles are more suitable for conjugation with antibodies because of lower non-specific sorption on a membrane.

Keywords

Immunochemistry; dot-blot; gold nanoparticles; LA-ICP-MS

Authors

VLČNOVSKÁ, M.; TVRDOŇOVÁ, M.; VACULOVIČOVÁ, M.; VACULOVIČ, T.

Released

2. 1. 2019

Publisher

Mendel University in Brno

ISBN

978-80-7509-597-8

Book

MendelNet 2018

Pages from

548

Pages to

552

Pages count

5

URL

https://mendelnet.cz/artkey/mnt-201801-0116_Utilization-of-antibody-nanoparticle-conjugates-as-a-tool-for-immunochemistry-with-ICP-MS-detection.php?back=/magno/mnt/2018/mn1.php?secid=4

Full text in the Digital Library

http://hdl.handle.net/11012/178390

BibTex

@inproceedings{BUT156248,
  author="Marcela {Vlčnovská} and Michaela {Tvrdoňová} and Markéta {Vaculovičová} and Tomáš {Vaculovič}",
  title="Utilization of antibody-nanoparticle conjugates as a tool for immunochemistry with ICP-MS detection",
  booktitle="MendelNet 2018",
  year="2019",
  pages="548--552",
  publisher="Mendel University in Brno",
  isbn="978-80-7509-597-8",
  url="https://mendelnet.cz/artkey/mnt-201801-0116_Utilization-of-antibody-nanoparticle-conjugates-as-a-tool-for-immunochemistry-with-ICP-MS-detection.php?back=/magno/mnt/2018/mn1.php?secid=4"
}