Publication detail

The Presence and Localization of G-Quadruplex Forming Sequences in the Domain of Bacteria

BARTAS, M. ČUTOVÁ, M. BRÁZDA, V. KAURA, P. ŠŤASTNÝ, J. KOLOMAZNÍK, J. COUFAL, J. GOSWAMI, P. ČERVEŇ, J. PEČINKA, P.

Original Title

The Presence and Localization of G-Quadruplex Forming Sequences in the Domain of Bacteria

Type

journal article in Web of Science

Language

English

Original Abstract

The role of local DNA structures in the regulation of basic cellular processes is an emerging field of research. Amongst local non-B DNA structures, the significance of G-quadruplexes was demonstrated in the last decade, and their presence and functional relevance has been demonstrated in many genomes, including humans. In this study, we analyzed the presence and locations of G-quadruplex-forming sequences by G4Hunter in all complete bacterial genomes available in the NCBI database. G-quadruplex-forming sequences were identified in all species, however the frequency differed significantly across evolutionary groups. The highest frequency of G-quadruplex forming sequences was detected in the subgroup Deinococcus-Thermus, and the lowest frequency in Thermotogae. G-quadruplex forming sequences are non-randomly distributed and are favored in various evolutionary groups. G-quadruplex-forming sequences are enriched in ncRNA segments followed by mRNAs. Analyses of surrounding sequences showed G-quadruplex-forming sequences around tRNA and regulatory sequences. These data point to the unique and non-random localization of G-quadruplex-forming sequences in bacterial genomes.

Keywords

G-quadruplex; bacteria; bioinformatics; deinococcus; G4Hunter

Authors

BARTAS, M.; ČUTOVÁ, M.; BRÁZDA, V.; KAURA, P.; ŠŤASTNÝ, J.; KOLOMAZNÍK, J.; COUFAL, J.; GOSWAMI, P.; ČERVEŇ, J.; PEČINKA, P.

Released

2. 5. 2019

ISBN

1420-3049

Periodical

MOLECULES

Year of study

24

Number

9

State

Swiss Confederation

Pages from

1

Pages to

13

Pages count

13

URL

https://www.mdpi.com/journal/molecules/special_issues/Gquadruplex_Microorganisms#published

BibTex

@article{BUT156781,
  author="BARTAS, M. and ČUTOVÁ, M. and BRÁZDA, V. and KAURA, P. and ŠŤASTNÝ, J. and KOLOMAZNÍK, J. and COUFAL, J. and GOSWAMI, P. and ČERVEŇ, J. and PEČINKA, P.",
  title="The Presence and Localization of G-Quadruplex Forming Sequences in the Domain of Bacteria",
  journal="MOLECULES",
  year="2019",
  volume="24",
  number="9",
  pages="1--13",
  doi="10.3390/molecules24091711",
  issn="1420-3049",
  url="https://www.mdpi.com/journal/molecules/special_issues/Gquadruplex_Microorganisms#published"
}