Publication detail

In-capillary derivatization with fluorescamine for the rapid determination of adamantane drugs by capillary electrophoresis with UV detection

PRAPATPONG, P. NUCHTAVORN, N. MACKA, M. SUNTORNSUK, L.

Original Title

In-capillary derivatization with fluorescamine for the rapid determination of adamantane drugs by capillary electrophoresis with UV detection

Type

journal article in Web of Science

Language

English

Original Abstract

In-capillary derivatization using fluorescamine as the labeling reagent was proposed to enhance the detectability of adamantine drugs (memantine, amantadine and rimantadine) by spectrophotometric detection. Fluorescamine and the drugs were delivered to the capillary electrophoresis instrument at a ratio of 10:1 by zone injection. The derivatization reaction occurred following the application of voltage (20kV). The derivatized products, hydrolyzed- fluorescamine and excess fluorescamine were separated in 7min using 100mM borate buffer (pH10.0) containing 0.1% w/v of Brij (R)-35 and 20% v/v of acetonitrile. Validation data showed good linearity (r(2)>0.98), precision (%RSDs<3.4), and accuracy (recoveries ranging from 98.0 to 102.0%). The detection and quantitation limits are in the range of 6.0-8.5 and 18-25M, respectively. The validation data is comparable to reported methods, however, the current method offers better precision with enhanced sensitivity (up to six times). Applications of the method show percent labeled amounts found in the studied samples within 100.6-109.3%, which complied with the United States Pharmacopeia limit (90.0-110.0%). The method was simple, rapid and, automated, which required no extra instrumentation or skillful operators.

Keywords

adamantine; fluorescamine; in-capillary derivatization; memantine; rimantadine

Authors

PRAPATPONG, P.; NUCHTAVORN, N.; MACKA, M.; SUNTORNSUK, L.

Released

31. 10. 2018

ISBN

1615-9306

Periodical

Journal of Separation Science

Year of study

41

Number

19

State

Federal Republic of Germany

Pages from

3764

Pages to

3771

Pages count

9

URL

https://onlinelibrary.wiley.com/doi/full/10.1002/jssc.201800591

BibTex

@article{BUT157233,
  author="Pornpan {Prapatpong} and Nantana {Nuchtavorn} and Miroslav {Macka} and Leena {Suntornsuk}",
  title="In-capillary derivatization with fluorescamine for the rapid determination of adamantane drugs by capillary electrophoresis with UV detection",
  journal="Journal of Separation Science",
  year="2018",
  volume="41",
  number="19",
  pages="3764--3771",
  doi="10.1002/jssc.201800591",
  issn="1615-9306",
  url="https://onlinelibrary.wiley.com/doi/full/10.1002/jssc.201800591"
}