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SEDLÁČEK, P. SLANINOVÁ, E. ENEV, V. KOLLER, M. NEBESAROVA, J. KRZYZANEK, V. SAMEK, O. MÁROVÁ, I. OBRUČA, S.
Original Title
What keeps polyhydroxyalkanoates in bacterial cells amorphous? A derivation from stress exposure experiments
Type
journal article in Web of Science
Language
English
Original Abstract
Polyhydroxyalkanoates (PHA) are storage polymers accumulated by numerous prokaryotes in form of intracellular granules. Native PHA granules are formed by amorphous polymer which reveals considerably higher elasticity and flexibility as compared to crystalline pure PHA polymers. The fact that bacteria store PHA in amorphous state has great biological consequences. It is not clear which mechanisms protect amorphous polymer in native granules from transition into thermodynamically favorable crystalline state. Here, we demonstrate that exposition of bacterial cells to particular stressors induces granules aggregation, which is the first but not sufficient condition for PHA crystallization. Crystallization of the polymer occurs only when the stressed bacterial cells are subsequently dried. The fact that both granules aggregation and cell drying must occur to induce crystallization of PHA indicates that both previously suggested hypotheses about mechanisms of stabilization of amorphous state of native PHA are valid and, in fact, both effects participate synergistically. It seems that the amorphous state of the polymer is stabilized kinetically by the low rate of crystallization in limited volume in small PHA granules and, moreover, water present in PHA granules seems to function as plasticizer protecting the polymer from crystallization, as confirmed experimentally for the first time by the present work.
Keywords
Polyhydroxyalkanoates; crystallization; Intracellular granules; Stress conditions
Authors
SEDLÁČEK, P.; SLANINOVÁ, E.; ENEV, V.; KOLLER, M.; NEBESAROVA, J.; KRZYZANEK, V.; SAMEK, O.; MÁROVÁ, I.; OBRUČA, S.
Released
8. 1. 2019
Publisher
Springer Berlin Heidelberg
ISBN
0175-7598
Periodical
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Year of study
103
Number
4
State
Federal Republic of Germany
Pages from
1905
Pages to
1917
Pages count
13
URL
https://link.springer.com/article/10.1007/s00253-018-09584-z
BibTex
@article{BUT157248, author="SEDLÁČEK, P. and SLANINOVÁ, E. and ENEV, V. and KOLLER, M. and NEBESAROVA, J. and KRZYZANEK, V. and SAMEK, O. and MÁROVÁ, I. and OBRUČA, S.", title="What keeps polyhydroxyalkanoates in bacterial cells amorphous? A derivation from stress exposure experiments", journal="APPLIED MICROBIOLOGY AND BIOTECHNOLOGY", year="2019", volume="103", number="4", pages="1905--1917", doi="10.1007/s00253-018-09584-z", issn="0175-7598", url="https://link.springer.com/article/10.1007/s00253-018-09584-z" }