Publication detail

Mouse model of congenital heart defects, dysmorphic facial features and intellectual developmental disorders as a result of nonfunctional CDK13

NOVÁKOVÁ, M. HAMPL, M. VRÁBEL, D. PROCHÁZKA, J. PETREZSELYOVA, S. PROCHÁZKOVÁ, M. SEDLÁČEK, R. KAVKOVÁ, M. ZIKMUND, T. KAISER, J. JUAN, H. FANN, M. BUCHTOVÁ, M. KOHOUTEK, J.

Original Title

Mouse model of congenital heart defects, dysmorphic facial features and intellectual developmental disorders as a result of nonfunctional CDK13

Type

journal article in Web of Science

Language

English

Original Abstract

Congenital heart defects, dysmorphic facial features and intellectual developmental disorders (CHDFFID) syndrome in humans was recently associated with mutation in CDK13 gene. In order to assess the loss of function of Cdk13 during mouse development, we employed gene trap knock-out allele in Cdk13 gene. Embryonic lethality of Cdk13-deficient animals was observed by the embryonic day 16.5, live embryos were observed on E15.5. At this stage, improper development of multiple organs has been documented, partly resembling defects observed in patients with mutated CDK13. In particular, slower brain development, incomplete palate formation, kidney failure accompanied by congenital heart defects were detected. Based on further analyses, the lethality at this stage is a result of heart failure most likely due to multiple heart defects followed by insufficient blood circulation resulting in multiple organs dysfunctions. Thus, Cdk13 knock-out mice might be a very useful model for further studies focused on delineating signaling circuits and molecular mechanisms underlying CHDFFID caused by mutation in CDK13 gene.

Keywords

cyclin-denpendent kinase, Transcription Regulation, development, cyclin-dependent kinase 13, Cyclin K

Authors

NOVÁKOVÁ, M.; HAMPL, M.; VRÁBEL, D.; PROCHÁZKA, J.; PETREZSELYOVA, S.; PROCHÁZKOVÁ, M.; SEDLÁČEK, R.; KAVKOVÁ, M.; ZIKMUND, T.; KAISER, J.; JUAN, H.; FANN, M.; BUCHTOVÁ, M.; KOHOUTEK, J.

Released

23. 7. 2019

Publisher

Frontiers Media SA

ISBN

2296-634X

Periodical

Frontiers in Cell and Developmental Biology

Year of study

7

Number

155

State

Swiss Confederation

Pages from

1

Pages to

19

Pages count

19

URL

https://www.frontiersin.org/articles/10.3389/fcell.2019.00155/full

Full text in the Digital Library

http://hdl.handle.net/11012/193257

BibTex

@article{BUT157858,
  author="Monika {Nováková} and Marek {Hampl} and David {Vrábel} and Jan {Procházka} and Silvia {Petrezselyova} and Michaela {Procházková} and Radislav {Sedláček} and Michaela {Kavková} and Tomáš {Zikmund} and Jozef {Kaiser} and Hsien-Chia {Juan} and Ming-Ji {Fann} and Marcela {Buchtová} and Jiří {Kohoutek}",
  title="Mouse model of congenital heart defects, dysmorphic facial features and intellectual developmental disorders as a result of nonfunctional CDK13",
  journal="Frontiers in Cell and Developmental Biology",
  year="2019",
  volume="7",
  number="155",
  pages="1--19",
  doi="10.3389/fcell.2019.00155",
  issn="2296-634X",
  url="https://www.frontiersin.org/articles/10.3389/fcell.2019.00155/full"
}