3D-Quantitative Morphometrics in Craniofacial Development by Xray Computed Microtomography

abstract

English

X-ray computed tomography is a widely used technique not only in medicine, but it has found its application also in industry and scientific fields including developmental biology. X-ray computed microtomography (microCT) allows to extract quantitative 3Dinformation about various objects with resolution down to 1 micrometer, and it is used for non-destructive testing, materials characterization and dimensional metrology. We have exploited these possibilities and applied them in craniofacial research. Modern developmental biology requires both qualitative and quantitative 3D-information about the studied objects, which is not provided by conventional 2D-imaging methods. Besides, the complexity of biological structures often requires a comprehensive methodology to compare shapes and sizes. Our approach is to apply procedures and analysis routinely used or developed for industrial microCT to address scientific questions in craniofacial research. This approach is based on the long-term experience of the CEITEC BUT microCT-research team in both, industrial and scientific applications. The connection of material and life sciences brings new possibilities in imaging, data processing and evaluation of 3D-biological models. In this work, we present 3D-structures that were analyzed by different approaches on selected examples from craniofacial research conducted on mouse embryos; e.g., wall thickness analysis of facial cartilage, shape comparisons of mouse heads, volume determination of ossification centers etc. A complete procedure consisting of staining, microCT-measurement and subsequent data processing is discussed.

Conference talk

ZIKMUND, T.; TESAŘOVÁ, M.; BUCHTOVÁ, M.; KAVKOVÁ, M.; HAMPL, M.; HRUBÁ, E.; KAUCKÁ, M.; ADAMEYKO, I.; KAISER, J.

3. 6. 2019

WILEY

0362-2525

JOURNAL OF MORPHOLOGY

280

United States of America

S243

S244

2

https://onlinelibrary.wiley.com/doi/abs/10.1002/jmor.21003