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AMIRMANSOOR, A. SÝS, M. SEDLÁČKOVÁ, E. FARAG, A. ADAM, V. PŘIBYL, J. RICHTERA, L.
Original Title
Application of the Enzymatic Electrochemical Biosensors for Monitoring Non-Competitive Inhibition of Enzyme Activity by Heavy Metals
Type
journal article in Web of Science
Language
English
Original Abstract
The inhibition effect of the selected heavy metals (Ag+, Cd2+, Cu2+, and Hg2+) on glucose oxidase (GOx) enzyme from Aspergillus niger (EC 1.1.3.4.) was studied using a new amperometric biosensor with an electrochemical transducer based on a glassy carbon electrode (GCE) covered with a thin layer of multi-wall carbon nanotubes (MWCNTs) incorporated with ruthenium(IV) oxide as a redox mediator. Direct adsorption of multi-wall carbon nanotubes (MWCNTs) and subsequent covering with Nafion((R)) layer was used for immobilization of Gox. The analytical figures of merit of the developed glucose (Glc) biosensor are sufficient for determination of Glc in body fluids in clinical analysis. From all tested heavy metals, mercury(II) has the highest inhibition effect. However, it is necessary to remember that cadmium and silver ions also significantly inhibit the catalytic activity of Gox. Therefore, the development of Gox biosensors for selective indirect determination of each heavy metal still represents a challenge in the field of bioelectroanalysis. It can be concluded that amperometric biosensors, differing in the utilized enzyme, could find their application in the toxicity studies of various poisons.
Keywords
glucose oxidase; heavy metals; amperometric biosensor; non-competitive inhibition
Authors
AMIRMANSOOR, A.; SÝS, M.; SEDLÁČKOVÁ, E.; FARAG, A.; ADAM, V.; PŘIBYL, J.; RICHTERA, L.
Released
3. 7. 2019
Publisher
MDPI
ISBN
1424-8220
Periodical
SENSORS
Year of study
19
Number
13
State
Swiss Confederation
Pages from
1
Pages to
15
Pages count
URL
https://www.mdpi.com/1424-8220/19/13/2939
Full text in the Digital Library
http://hdl.handle.net/11012/180521
BibTex
@article{BUT158050, author="Amirmansoor {Ashrafi} and Milan {Sýs} and Eliška {Dostálová} and Amir Shaaban {Farag} and Vojtěch {Adam} and Jan {Přibyl} and Lukáš {Richtera}", title="Application of the Enzymatic Electrochemical Biosensors for Monitoring Non-Competitive Inhibition of Enzyme Activity by Heavy Metals", journal="SENSORS", year="2019", volume="19", number="13", pages="1--15", doi="10.3390/s19132939", issn="1424-8220", url="https://www.mdpi.com/1424-8220/19/13/2939" }