Publication detail

Cisplatin enhances cell stiffness and decreases invasiveness rate in prostate cancer cells by actin accumulation

RAUDENSKÁ, M. KRATOCHVÍLOVÁ, M. VIČAR, T. GUMULEC, J. BALVAN, J. POLANSKÁ, H. PŘIBYL, J. MASAŘÍK, M.

Original Title

Cisplatin enhances cell stiffness and decreases invasiveness rate in prostate cancer cells by actin accumulation

Type

journal article in Web of Science

Language

English

Original Abstract

We focused on the biomechanical and morphological characteristics of prostate cancer cells and their changes resulting from the effect of docetaxel, cisplatin, and long-term zinc supplementation. Cell population surviving the treatment was characterized as follows: cell stiffness was assessed by atomic force microscopy, cell motility and invasion capacity were determined by colony forming assay, wound healing assay, coherence-controlled holographic microscopy, and real-time cell analysis. Cells of metastatic origin exhibited lower height than cells derived from the primary tumour. Cell dry mass and CAV1 gene expression followed similar trends as cell stiffness. Docetaxel- and cisplatin-surviving cells had higher stiffness, and decreased motility and invasive potential as compared to non-treated cells. This effect was not observed in zinc(II)-treated cells. We presume that cell stiffness changes may represent an important overlooked effect of cisplatin-based anti-cancer drugs. Atomic force microscopy and confocal microscopy data images used in our study are available for download in the Zenodo repository (https://zenodo.org/, Digital Object Identifiers:10.5281/zenodo.1494935).

Keywords

docetaxel, cisplatin, cell stifness, zinc, coherence-controlled, atomic force microscopy, holographic microscopy

Authors

RAUDENSKÁ, M.; KRATOCHVÍLOVÁ, M.; VIČAR, T.; GUMULEC, J.; BALVAN, J.; POLANSKÁ, H.; PŘIBYL, J.; MASAŘÍK, M.

Released

7. 2. 2019

Publisher

NATURE PUBLISHING GROUP

Location

MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND

ISBN

2045-2322

Periodical

Scientific Reports

Year of study

9

Number

1

State

United Kingdom of Great Britain and Northern Ireland

Pages from

1

Pages to

11

Pages count

11

URL

https://www.nature.com/articles/s41598-018-38199-7

Full text in the Digital Library

http://hdl.handle.net/11012/184678

BibTex

@article{BUT158198,
  author="Martina {Raudenská} and Monika {Kratochvílová} and Tomáš {Vičar} and Jaromír {Gumulec} and Jan {Balvan} and Hana {Polanská} and Jan {Přibyl} and Michal {Masařík}",
  title="Cisplatin enhances cell stiffness and decreases invasiveness rate in prostate cancer cells by actin accumulation",
  journal="Scientific Reports",
  year="2019",
  volume="9",
  number="1",
  pages="1--11",
  doi="10.1038/s41598-018-38199-7",
  issn="2045-2322",
  url="https://www.nature.com/articles/s41598-018-38199-7"
}