Publication detail

CdS quantum dots-based immunoassay combined with particle imprinted polymer technology and laser ablation ICP-MS as a versatile tool for protein detection

VANĚČKOVÁ, T. BEZDĚKOVÁ, J. TVRDOŇOVÁ, M. VLČNOVSKÁ, M. NOVOTNÁ, V. NEUMAN, J. ŠTOSSOVÁ, A. KANICKÝ, V. ADAM, V. VACULOVIČOVÁ, M. VACULOVIČ, T.

Original Title

CdS quantum dots-based immunoassay combined with particle imprinted polymer technology and laser ablation ICP-MS as a versatile tool for protein detection

Type

journal article in Web of Science

Language

English

Original Abstract

For the first time, the combination of molecularly imprinted polymer (MIP) technology with laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is presented with focus on an optimization of the LA-ICP-MS parameters such as laser beam diameter, laser beam fluence, and scan speed using CdS quantum dots (QDs) as a template and dopamine as a functional monomer. A non-covalent imprinting approach was employed in this study due to the simplicity of preparation. Simple oxidative polymerization of the dopamine that creates the self-assembly monolayer seems to be an ideal choice. The QDs prepared by UV light irradiation synthesis were stabilized by using mercaptosuccinic acid. Formation of a complex of QD-anti body and QD-antibody-antigen was verified by using capillary electrophoresis with laser-induced fluorescence detection. QDs and antibody were connected together via an affinity peptide linker. LA-ICP-MS was employed as a proof-of-concept for detection method of two types of immunoassay: 1) antigen extracted from the sample by MIP and subsequently overlaid/immunoreacted by QD-labelled antibodies, 2) complex of antigen, antibody, and QD formed in the sample and subsequently extracted by MIP. The first approach provided higher sensitivity (MIP/NIP), however, the second demonstrated higher selectivity. A mixture of proteins with size in range 10-250 kDa was used as a model sample to demonstrate the capability of both approaches for detection of IgG in a complex sample.

Keywords

CdS quantum dots (QDs); molecularly imprinted polymer (MIP); laser ablation ICP-MS

Authors

VANĚČKOVÁ, T.; BEZDĚKOVÁ, J.; TVRDOŇOVÁ, M.; VLČNOVSKÁ, M.; NOVOTNÁ, V.; NEUMAN, J.; ŠTOSSOVÁ, A.; KANICKÝ, V.; ADAM, V.; VACULOVIČOVÁ, M.; VACULOVIČ, T.

Released

14. 8. 2019

Publisher

Springer Nature

ISBN

2045-2322

Periodical

Scientific Reports

Year of study

9

Number

1

State

United Kingdom of Great Britain and Northern Ireland

Pages from

1

Pages to

9

Pages count

9

URL

http://link.springer.com/article/10.1038/s41598-019-48290-2

Full text in the Digital Library

http://hdl.handle.net/11012/180684

BibTex

@article{BUT158577,
  author="Tereza {Vaněčková} and Jaroslava {Bezděková} and Michaela {Kuchynka} and Marcela {Vlčnovská} and Veronika {Novotná} and Jan {Neuman} and Aneta {Štossová} and Viktor {Kanický} and Vojtěch {Adam} and Markéta {Vaculovičová} and Tomáš {Vaculovič}",
  title="CdS quantum dots-based immunoassay combined with particle imprinted polymer technology and laser ablation ICP-MS as a versatile tool for protein detection",
  journal="Scientific Reports",
  year="2019",
  volume="9",
  number="1",
  pages="1--9",
  doi="10.1038/s41598-019-48290-2",
  issn="2045-2322",
  url="http://link.springer.com/article/10.1038/s41598-019-48290-2"
}