Publication detail

Laser-Induced Breakdown Spectroscopy as a Novel Readout Method for Nanoparticle-Based Immunoassays

MODLITBOVÁ, P. FARKA, Z. PASTUCHA, M. POŘÍZKA, P. NOVOTNÝ, K. SKLÁDAL, P. KAISER, J.

Original Title

Laser-Induced Breakdown Spectroscopy as a Novel Readout Method for Nanoparticle-Based Immunoassays

Type

conference proceedings

Language

English

Original Abstract

Laser-Induced Breakdown Spectroscopy (LIBS) was examined as a novel method to provide the readout of microtiter plate immunoassays involving nanoparticles (NP). The so-called Tag-LIBS, i.e. a sensitive detection of specific biomarkers, was applied to detect the NP labels using the nanosecond ablation sampling. The NP labels were examined from the bottom of the standard 96-well microtiter plate. Thanks to the well-known flexibility in the LIBS instrumentation, the plasma emission collection as well as the focusing optics can be rebuilt into a collinear arrangement. The presented experiments proved the suitable detection of silver NPs from the microtiter plate’s bottom without any negative effects on the NPs’ qualitative and quantitative analysis. Subsequently, a sandwich immunoassay that detects human serum albumin was successfully developed, using streptavidin-coated AgNP labels. The assay provided a detection limit of 10 ng·mL−1 which is comparable with the fluorescence-based readout. The main advantage of LIBS readout is that it enables a detection of any type of NP labels independent on their fluorescence or catalytic properties. Owing to the immediate signal response, a relatively simple instrumentation enabling the automation, and the capability of multi-elemental analyses, LIBS technique offers a promising and fast alternative to other readout techniques allowing a multiplex detection of various biomarkers.

Keywords

LIBS; immunoassay; nanoparticles; streptavidin; albumin

Authors

MODLITBOVÁ, P.; FARKA, Z.; PASTUCHA, M.; POŘÍZKA, P.; NOVOTNÝ, K.; SKLÁDAL, P.; KAISER, J.

Released

23. 10. 2019

Publisher

Spektroskopická společnost Jana Marka Marci

Location

Ke Karlovu 2027/3, 120 00 Praha 2 - Nové Město

ISBN

978-80-88195-13-9

Book

EMSLIBS 2019 Book of abstracts

Pages from

236

Pages to

236

Pages count

293

URL

https://emslibs.com/wp-content/uploads/2019/09/b3.2.pdf

BibTex

@proceedings{BUT159094,
  editor="MODLITBOVÁ, P. and FARKA, Z. and PASTUCHA, M. and POŘÍZKA, P. and NOVOTNÝ, K. and SKLÁDAL, P. and KAISER, J.",
  title="Laser-Induced Breakdown Spectroscopy as a Novel Readout Method for Nanoparticle-Based Immunoassays",
  year="2019",
  pages="236--236",
  publisher="Spektroskopická společnost Jana Marka Marci",
  address="Ke Karlovu 2027/3, 120 00 Praha 2 - Nové Město",
  isbn="978-80-88195-13-9",
  url="https://emslibs.com/wp-content/uploads/2019/09/b3.2.pdf"
}