Signaling Pathway for Butanol Production in Clostridium beijerinckii NRRL B-598

conference paper

English

The unstable price of oil in the last decade is the reason for an increased interest in the sustainable production of biofuels. Microbial production is again becoming a competitive way of generating butanol, and it is a suitable alternative to the chemical production of oil. Still, there is plenty of room for the development of highly efficient phenotypes of various solvent-producing organisms. The key lies in the utilisation of systems biology and its models that can help usto fully understand the whole process on a molecular level in the selected organism, and to identify the genomic regions responsible for the desired phenotype features. Here, we present a dynamic model of butanol production in a promising solvent producer, Clostridium beijerinckii NRRL B-598. We utilised a wide range of data we collected over the last two years, including RNA-Seq gene expression, cell viability measured with flow cytometry, and metabolic activity measured with HPLC, to reconstruct a pathway involved in butanol production. Although solventogenic species are extensively studied organisms, we bring the first pathway of the strain C. beijerinckii NRRL B-598. We used an open platform, Cell Collective (cellcollective.org), where the model is publicly available under the name ‘Signalling Pathway for Butanol Production in Clostridium beijerinckii NRRL B-598’, version 1.1. To determine the properties of the pathway, we performed a series of static and dynamic analyses. The presented pathway is an ideal approach for exploring the possibilities of biofuel overproduction as it enables the activation or inactivation of individual genes and metabolites involved in the production of butanol. Not only can the acquired knowledge help with future engineering of the strain, but the results can be generalised for the microbial production of butanol in related species.

signaling pathway; clostridium beijerinckii; butanol

MUSILOVÁ, J.; JUREČKOVÁ, K.; KOLEK, J.; PROVAZNÍK, I.; PATÁKOVÁ, P.; SEDLÁŘ, K.

29. 10. 2019

Czech Society of Industrial Chemistry

978-80-88307-00-6

Proceedings of the 7th International Conference on Chemical Technology

46

52

7

https://www.icct.cz/AngiologyKlon-ICCT/media/system/2019/ICCT-2019-Proceedings.pdf