Evolutionary engineering approach for enhancement of growth characteristics and producing capacity of selected PHA producing microorganisms

abstract

English

Polyhydroxyalkanoates (PHAs) are microbial polymers which could replace traditional petrochemical plastics due to their favorable properties such as biodegradability and biocompatibility. Application of approaches of evolutionary engineering could be used to obtain microorganisms producing PHAs with required properties. Selected microorganisms Cupriavidus necator H16 (CCM 3726) and Halomonas halophila (CCM 3662) have been exposed to several biotechnologically relevant stressors. C. necator H16 was exposed to presence of NaCl causing osmotic stress which represented environmental stress factor, to copper ions representing anthropogenic pollutant and to levulinic acid. Strain H. halophila was exposed to acetic and also to levulinic acid; components of hydrolysate of lignocellulosic biomass. Evolutionary experiments were provided using multiple serial transfers of cell cultures in Erlenmeyer flasks after 48 hours of cultivation. Except basic screening, selected preserved passages were characterized considering their potential of PHAs accumulation, effectivity of utilization of organic acids, testing of robustness and other differences between evolved strains and wild-type strains.

evolutionary engineering, polyhydroxyalkanoates, Cupriavidus necator H16, Halomonas halophila

NOVÁČKOVÁ, I.; KUČERA, D.; POŘÍZKA, J.; SEDLÁČEK, P.; PERNICOVÁ, I.; OBRUČA, S.

21. 11. 2019

Vysoké učení technické v Brně, Fakulta chemická

Brno

978-80-214-5807-9

Studentská odborná konference Chemie je život 2019 - Sborník abstraktů

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