Publication detail

CdSe QD biosynthesis in yeast using tryptone-enriched media and their conjugation with a peptide hecate for bacterial detection and killing

SUR, V. KOMÍNKOVÁ, M. BUCHTOVÁ, Ž. DOLEŽELÍKOVÁ, K. ZÍTKA, O. MOULICK, A.

Original Title

CdSe QD biosynthesis in yeast using tryptone-enriched media and their conjugation with a peptide hecate for bacterial detection and killing

Type

journal article in Web of Science

Language

English

Original Abstract

The physical and chemical synthesis methods of quantum dots (QDs) are generally unfavorable for biological applications. To overcome this limitation, the development of a novel "green" route to produce highly-fluorescent CdSe QDs constitutes a promising substitute approach. In the present work, CdSe QDs were biosynthesized in yeast Saccharomyces cerevisiae using a novel method, where we showed for the first time that the concentration of tryptone highly affects the synthesis process. The optimum concentration of tryptone was found to be 25 g/L for the highest yield. Different methods were used to optimize the QD extraction from yeast, and the best method was found to be by denaturation at 80 degrees C along with an ultrasound needle. Multiple physical characterizations including transmission electron microscopy (TEM), dynamic light scattering (DLS), energy-dispersive X-ray spectroscopy (EDX), and spectrophotometry confirmed the optical features size and shape distribution of the QDs. We showed that the novel conjugate of the CdSe QDs and a cell-penetrating peptide (hecate) can detect bacterial cells very efficiently under a fluorescent microscope. The conjugate also showed strong antibacterial activity against vancomycin-resistant Staphylococcus aureus (VRSA), methicillin-resistant Staphylococcus aureus (MRSA), and Escherichia coli, which may help us to cope with the problem of rising antibiotic resistance.

Keywords

biosynthesis; tryptone; QDs; cell-penetrating peptide; antibacterial

Authors

SUR, V.; KOMÍNKOVÁ, M.; BUCHTOVÁ, Ž.; DOLEŽELÍKOVÁ, K.; ZÍTKA, O.; MOULICK, A.

Released

31. 10. 2019

Publisher

MDPI

ISBN

2079-4991

Periodical

Nanomaterials

Year of study

9

Number

10

State

Swiss Confederation

Pages from

1

Pages to

15

Pages count

15

URL

https://www.mdpi.com/2079-4991/9/10/1463

Full text in the Digital Library

http://hdl.handle.net/11012/184080

BibTex

@article{BUT160566,
  author="Vishma Pratap {Sur} and Markéta {Komínková} and Žaneta {Buchtová} and Kristýna {Doleželíková} and Ondřej {Zítka} and Amitava {Moulick}",
  title="CdSe QD biosynthesis in yeast using tryptone-enriched media and their conjugation with a peptide hecate for bacterial detection and killing",
  journal="Nanomaterials",
  year="2019",
  volume="9",
  number="10",
  pages="1--15",
  doi="10.3390/nano9101463",
  issn="2079-4991",
  url="https://www.mdpi.com/2079-4991/9/10/1463"
}