Publication detail

Study of Metabolic Adaptation of Red Yeasts to Waste Animal Fat Substrate

SZOTKOWSKI, M. BYRTUSOVÁ, D. NĚMCOVÁ, A. VYSOKÁ, M. RAPTA, M. SHAPAVAL, V. MÁROVÁ, I.

Original Title

Study of Metabolic Adaptation of Red Yeasts to Waste Animal Fat Substrate

Type

journal article in Web of Science

Language

English

Original Abstract

Carotenogenic yeasts are non-conventional oleaginous microorganisms capable to utilize various waste substrates. In this work 4 red yeast strains (Rhodotorula, Cystofilobasidium and Sporobolomyces sp.) were cultivated in media containing crude, emulsified and enzymatically hydrolysed animal waste fat, compared with glucose and glycerol as single C-sources. Cell morphology (cryo-SEM, TEM), production of biomass, lipase, biosurfactants, lipids (GC/FID) carotenoids, ubiquinone, ergosterol (HPLC/PDA) in yeast cells was studied depending on medium composition, C-source and C/N ratio. All studied strains are able to utilize solid and processed fat. Biomass production at C/N=13 was higher on emulsified/hydrolysed fat than on glucose/glycerol. Production of lipids and lipidic metabolites was enhanced for several times on fat; the highest yields of carotenoids (24.8 mg/l) and lipids (54.5%/CDW) were found in S.pararoseus. Simultaneous induction of lipase and biosurfactants was observed on crude fat substrate. Increased C/N ratio (13-100) led to higher biomass production in fat media. Production of total lipids increased in all strains to C/N 50. Oppositely, production of carotenoids, ubiquinone and ergosterol dramatically decreased with increased C/N in all strains. Compounds accumulated in stressed red yeasts are having great application potential and can result from valorization of animal waste fat in the biorefinery concept.

Keywords

carotenogenic yeasts; lipids; carotenoids; lipase; biosurfactants; ubiquinone; ergosterol

Authors

SZOTKOWSKI, M.; BYRTUSOVÁ, D.; NĚMCOVÁ, A.; VYSOKÁ, M.; RAPTA, M.; SHAPAVAL, V.; MÁROVÁ, I.

Released

19. 11. 2019

Publisher

MDPI

ISBN

2076-2607

Periodical

Microorganisms

Year of study

7

Number

11

State

Swiss Confederation

Pages from

1

Pages to

28

Pages count

28

URL

https://www.mdpi.com/2076-2607/7/11/578

Full text in the Digital Library

http://hdl.handle.net/11012/184660

BibTex

@article{BUT160868,
  author="Martin {Szotkowski} and Dana {Byrtusová} and Andrea {Němcová} and Marie {Vysoká} and Marek {Rapta} and Volha {Shapaval} and Ivana {Márová}",
  title="Study of Metabolic Adaptation of Red Yeasts to Waste Animal Fat Substrate",
  journal="Microorganisms",
  year="2019",
  volume="7",
  number="11",
  pages="1--28",
  doi="10.3390/microorganisms7110578",
  issn="2076-2607",
  url="https://www.mdpi.com/2076-2607/7/11/578"
}