Publication detail

Multi-electrode array with a planar surface for cell patterning by microprinting

SLAVÍK, J. SKOPALÍK, J. PROVAZNÍK, I. HUBÁLEK, J.

Original Title

Multi-electrode array with a planar surface for cell patterning by microprinting

Type

journal article in Web of Science

Language

English

Original Abstract

Multielectrode arrays (MEAs) are devices for non-invasive electrophysiological measurements of cell populations. This paper describes a novel fabrication method of MEAs with a fully planar surface. The surface of the insulation layer and the surface of the electrodes were on one plane; we named this device the planar MEA (pMEA). The main advantage of the pMEA is that it allows uniform contact between the pMEA surface and a substrate for positioning of microfluidic channels or microprinting of a cell adhesive layer. The fabrication of the pMEA is based on a low adhesive Au sacrificial peel-off layer. In divergence from conventional MEAs with recessed electrodes, the electrodes of the pMEA lead across the sloped edge of the insulation layer. To make this, the profile of the edge of the insulation layer was measured and the impedance of the planar electrodes was characterized. The impedance of the pMEA was comparable with the impedance of conventional MEA electrodes. The pMEA was tested for patterning HL-1 cells with a combination of imprinting fibronectin and coating by antifouling poly (l-lysine)-graft-poly(ethylene glycol) (PLL-g-PEG). The HL-1 cells remained patterned even at full confluency and presented spontaneous and synchronous beating activity.

Keywords

multielectrode array; microelectrode array; sacrificial layer; peel-off; HL-1 cells; microprinting; antifouling agent

Authors

SLAVÍK, J.; SKOPALÍK, J.; PROVAZNÍK, I.; HUBÁLEK, J.

Released

5. 12. 2019

Publisher

MDPI

ISBN

1424-8220

Periodical

SENSORS

Year of study

19

Number

24

State

Swiss Confederation

Pages from

1

Pages to

11

Pages count

11

URL

https://www.mdpi.com/1424-8220/19/24/5379

Full text in the Digital Library

http://hdl.handle.net/11012/184124

BibTex

@article{BUT161298,
  author="Jan {Slavík} and Josef {Skopalík} and Valentine {Provazník} and Jaromír {Hubálek}",
  title="Multi-electrode array with a planar surface for cell patterning by microprinting",
  journal="SENSORS",
  year="2019",
  volume="19",
  number="24",
  pages="1--11",
  doi="10.3390/s19245379",
  issn="1424-8220",
  url="https://www.mdpi.com/1424-8220/19/24/5379"
}