Publication detail

Monitoring the uptake and toxicity of nanoparticles in plants using laser-induced breakdown spectroscopy

POŘÍZKA, P. MODLITBOVÁ, P. STŘÍTEŽSKÁ, S. KAISER, J.

Original Title

Monitoring the uptake and toxicity of nanoparticles in plants using laser-induced breakdown spectroscopy

Type

conference proceedings

Language

English

Original Abstract

The increasing production and utilization of nanoparticles (NPs) across applications led to their transfer into the environment, typically into the water sources. The most common NPs are based on noble metals (AgNPs, AuNPs) or metal oxides (ZnO NPs, TiO 2 NPs). However, there are also NPs with unique optical properties such as nanocrystals of chalcogens (CdTe, CdSe) so-called Quantum dots (QDs) and nanocrystals containing yttrium (NaYF 4 ); so-called photon up-conversion nanoparticles (UCNPs). Both types of luminescent NPs became widely used as the molecular probes in biomedical and biological applications such as biolabeling, bioimaging, biotargeting, and drug delivery. They can replace the traditional fluorescent dyes (i.e. acridine orange, eosin). This has naturally triggered an intense investigation of their toxicity and impact on living organisms. Common toxicity tests are based on the exposure of model organisms (various plants in our case) to selected contaminants (QDs or UCNPs in this study) with established toxicity end-points (the plant growth or the plant biomass decrease). Such a routine could be followed by an investigation of the bioaccumulation of test compounds in the whole plant or its part. The investigation is done using the standard techniques of analytical chemistry e.g. ICP-OES (Inductively coupled plasma - optical emission spectrometry) or AAS (Atomic absorption spectroscopy) after the sample decomposition. However, such approaches fail to provide any information about the distribution of NPs with respect to their position. Laser-Induced Breakdown Spectroscopy (LIBS) has been successfully adapted to toxicological routine as a tool providing large-scale qualitative maps of complete plants (root, shoots, and leaves/fronds). In our talk, we will summarize recent advances and applications of LIBS in monitoring various NPs within the selected aquatic plants as duckweed ( Lemna minor L.) or terrestrial plants exposed in hydroponic conditions as radish ( Raphanus sativus L.) .

Keywords

Laser spectroscopy, toxicology, nanoparticles

Authors

POŘÍZKA, P.; MODLITBOVÁ, P.; STŘÍTEŽSKÁ, S.; KAISER, J.

Released

17. 10. 2019

Publisher

Spektroskopická společnost Jana Marka Marci

Location

Ke Karlovu 2027/3, 120 00 Praha 2 - Nové Město

Pages count

293

URL

http://libs.ceitec.cz/files/281/213.pdf

BibTex

@proceedings{BUT161316,
  editor="Pavel {Pořízka} and Pavlína {Modlitbová} and Sára {Střítežská} and Jozef {Kaiser}",
  title="Monitoring the uptake and toxicity of nanoparticles in plants using laser-induced breakdown spectroscopy",
  year="2019",
  pages="293",
  publisher="Spektroskopická společnost Jana Marka Marci",
  address="Ke Karlovu 2027/3, 120 00 Praha 2 - Nové Město",
  url="http://libs.ceitec.cz/files/281/213.pdf"
}