Publication detail

Interaction of selenite with metallothionein studied by Brdika reaction

SLAVÍK, J. TRNKOVÁ, L. HUBÁLEK, J.

Original Title

Interaction of selenite with metallothionein studied by Brdika reaction

Type

journal article in Web of Science

Language

English

Original Abstract

Metallothionein II (MT) is a cytosolic, ubiquitous, low-molecular-weight protein present in various tissues of mammals and non-mammals. MTs have a high content of thiol groups (-SH) which can bind mineral micronutrients and xenobiotic heavy metals. In this study, we investigated the electrode processes of MT at a mercury electrode in the presence of sodium selenite by means of the Brdika reaction in the differential pulse voltammetric mode. In Brdika solution containing ammonium buffer and [Co(NH3)(6)]Cl-3, the substance that provides catalytic reactions with proteins, interactions between MT and sodium selenite were analyzed via the hydrogen evolution catalytic signals. The reaction of -SH groups of MT with [Co(H2O)(6)](2+) complex ions on the mercury surface results into the catalytic peak Cat2. It was found that selenite ions are active in the Brdika reaction and yield also the catalytic peak of hydrogen evolution Cat2. With the increasing concentration of sodium selenite, cobalt (Co) in MT is replaced by selenium (Se). When selenite concentration increases above the MT binding capacity, i.e., MT cannot bind more Se, only selenite ions can be responsible for Cat2 signals. From our results, we conclude that Brdika reaction is a suitable method to study MT binding properties and to evaluate the influence of sodium selenite. To elucidate the experimental results a new interpretation leading to complete description of the mechanism is presented. [GRAPHICS] .

Keywords

Metallothionein; Selenite; Brdika reaction; Catalytic signals; Hanging mercury drop electrode; Differential pulse voltammetry

Authors

SLAVÍK, J.; TRNKOVÁ, L.; HUBÁLEK, J.

Released

1. 3. 2019

Publisher

SPRINGER WIEN

Location

WIEN

ISBN

1434-4475

Periodical

Monatshefte für Chemie

Year of study

150

Number

3

State

Republic of Austria

Pages from

469

Pages to

475

Pages count

7

URL

BibTex

@article{BUT161317,
  author="Jan {Slavík} and Libuše {Trnková} and Jaromír {Hubálek}",
  title="Interaction of selenite with metallothionein studied by Brdika reaction",
  journal="Monatshefte für Chemie",
  year="2019",
  volume="150",
  number="3",
  pages="469--475",
  doi="10.1007/s00706-019-02397-4",
  issn="1434-4475",
  url="https://link.springer.com/article/10.1007/s00706-019-02397-4"
}