Publication detail

The effect of different fatty acid sources on wound healing in rats assessed by matrix-assisted-laser-desorption-ionization mass-spectroscopy-imaging

KOMPRDA, T. SLÁDEK, Z. ŠVEHLOVÁ, V. LACKOVÁ, Z. GURÁŇ, R. DO, T. WIJACKI, J. BUCHTOVÁ, M. NEUWIRTHOVÁ, J. GÁL, B. ADAM, V. ZÍTKA, O.

Original Title

The effect of different fatty acid sources on wound healing in rats assessed by matrix-assisted-laser-desorption-ionization mass-spectroscopy-imaging

Type

journal article in Web of Science

Language

English

Original Abstract

The objective of the present study was to compare the effects of dietary oils containing polyunsaturated fatty acids (PUFA) n-3 and n-6, respectively, on cutaneous wound healing in rats, and to demonstrate the usefulness of the matrix-assisted-laser-desorption-ionization mass spectroscopy-imaging (MALDI MSI) method in this type of experiment. Superiority of PUFA n-3 in this context was the tested hypothesis. Four groups of male Wistar rats by twelve animals each were fed a diet with added 5% of palm oil (P; control), fish oil (F), Schizochytrium microalga oil (Sch) and safflower oil (S), respectively, for eight weeks. Consequently, dorsal full-thickness cutaneous excisions were performed, and selected markers of wound healing were evaluated 18 days post excision. The median of signal intensity corresponding to an amount of collagen alpha-1 (III) fragment, quantified using MALDI MSI, decreased in a sequence P > F > Sch > S (P < 0.001). Using haematoxylin-eosin staining of the histological preparations, semiquantitatively assessed epithelium height tended to decrease in the order of P > S > Sch > F; the wound extent in the sequence of P > Sch > S > F; and the sequence of the progress of neoangiogenesis was assessed as S > P > F approximate to Sch. It was concluded that the tested hypothesis was confirmed only partially: PUFA n-3 showed better results regarding the wound extent, but were inferior in terms of epithelium height and progress of neo-angiogenesis. This was the first time MALDI MSI was successfully employed for evaluating skin wound healing in a rat model.

Keywords

Docosahexaenoic acid; eicosapentaenoic acid; Schizochytrium oil; reepithelialization; MALDI MSI; collagen alpha-1(III)

Authors

KOMPRDA, T.; SLÁDEK, Z.; ŠVEHLOVÁ, V.; LACKOVÁ, Z.; GURÁŇ, R.; DO, T.; WIJACKI, J.; BUCHTOVÁ, M.; NEUWIRTHOVÁ, J.; GÁL, B.; ADAM, V.; ZÍTKA, O.

Released

1. 12. 2019

Publisher

University of Veterinary and Pharmaceutical Sciences in Brno

ISBN

0001-7213

Periodical

ACTA VETERINARIA BRNO

Year of study

88

Number

4

State

Czech Republic

Pages from

443

Pages to

451

Pages count

9

URL

https://actavet.vfu.cz/88/4/0443/

Full text in the Digital Library

http://hdl.handle.net/11012/184648

BibTex

@article{BUT162414,
  author="Tomáš {Komprda} and Zbyšek {Sládek} and Veronika {Švehlová} and Zuzana {Lacková} and Roman {Guráň} and Tomáš {Do} and Jan {Wijacki} and Marcela {Buchtová} and Jana {Neuwirthová} and Břetislav {Gál} and Vojtěch {Adam} and Ondřej {Zítka}",
  title="The effect of different fatty acid sources on wound healing in rats assessed by matrix-assisted-laser-desorption-ionization mass-spectroscopy-imaging",
  journal="ACTA VETERINARIA BRNO",
  year="2019",
  volume="88",
  number="4",
  pages="443--451",
  doi="10.2754/avb201988040443",
  issn="0001-7213",
  url="https://actavet.vfu.cz/88/4/0443/"
}