Publication detail

Study of Echinacea Content Substances

ZEMANOVÁ, J. VÍTOVÁ, E. ĎUBAŠÁKOVÁ, M. VESPALCOVÁ, M.

Original Title

Study of Echinacea Content Substances

Type

conference paper

Language

English

Original Abstract

In this study, high-performance liquid chromatography was used for a quantitative determination of caffeic acid and its derivatives including chlorogenic acid, cichoric acid and echinacoside as the content compounds present in the alcoholic extracts of Echinacea species. To the most significant Echinacea species, Echinacea purpurea, Echinacea angustifolia and Echinacea pallida belong. Their importance especially lies in the content of a wide range of effective compounds such as alkamides, caffeic acid derivatives, polyacetylenes and polyenes, polysaccharides and flavanoids. Echinacea species show antioxidative, antibacterial, antiviral and antifungal properties. More recently, Echinacea has been studied even as an inhibition of HIV replication. Echinacea occupies a fundamental place between herbal plants, that have been more and more used in a treatment of various diseases. In the experimental part, the determination of the total compounds content and echinacoside content in Echinacea extracts was made. The extracts were produced by pressurized solvent extraction from the plant Echinacea purpurea under various conditions. Extraction at 80 °C and 15 kPa using a mixture of ethanol/water (40/60, v/v) as a solvent system gave the most concentrated samples. Extraction at 60 °C and 15 kPa using a mixture of ethanol/water (40/60, v/v) as a solvent system is proper for a leaf and bloom material, while root material is better to be extracted in ethanol/water (60/40, v/v). In case of extraction at 40 °C and 15 kPa using a solvent system of ethanol/water (40/60, v/v), more concentrated samples were obtained from roots. In the case of leaves and blooms, it is good to use ethanol/water (60/40, v/v). The highest content of echinacoside was observed in the root extracts as well as leaf-bloom extracts prepared in ethanol/water = 40/60 at 80 °C and 15 kPa.

Keywords

Echinacea species, content substances, caffeic acid derivatives, echinacoside, HPLC

Authors

ZEMANOVÁ, J.; VÍTOVÁ, E.; ĎUBAŠÁKOVÁ, M.; VESPALCOVÁ, M.

Released

15. 10. 2019

Publisher

Univerzita Pardubice

Location

Pardubice

ISBN

978-80-7560-245-9

Book

19th International Nutrition & Diagnostics Conference - Book of Proceedings

Edition number

1

Pages from

98

Pages to

98

Pages count

1

URL

http://www.radanal.cz/cs/

BibTex

@inproceedings{BUT162622,
  author="Jana {Zemanová} and Eva {Vítová} and Mária {Badárová} and Milena {Vespalcová}",
  title="Study of Echinacea Content Substances",
  booktitle="19th International Nutrition & Diagnostics Conference - Book of Proceedings",
  year="2019",
  number="1",
  pages="98--98",
  publisher="Univerzita Pardubice",
  address="Pardubice",
  isbn="978-80-7560-245-9",
  url="http://www.radanal.cz/cs/"
}