Publication detail

The Rhizophagus irregularis Genome Encodes Two CTR Copper Transporters That Mediate Cu Import Into the Cytosol and a CTR-Like Protein Likely Involved in Copper Tolerance

GOMEZ-GALLEGO, T. BENABDELLAH, K. MERLOS RODRIGO, M. JIMENEZ JIMENEZ, A. ALCON, C. BERTHOMIEU, P. FERROL, N.

Original Title

The Rhizophagus irregularis Genome Encodes Two CTR Copper Transporters That Mediate Cu Import Into the Cytosol and a CTR-Like Protein Likely Involved in Copper Tolerance

Type

journal article in Web of Science

Language

English

Original Abstract

Arbuscular mycorrhizal fungi increase fitness of their host plants under Cu deficient and toxic conditions. In this study, we have characterized two Cu transporters of the CTR family (RiCTR1 and RiCTR2) and a CTR-like protein (RiCTR3A) of Rhizophagus irregularis. Functional analyses in yeast revealed that RiCTR1 encodes a plasma membrane Cu transporter, RiCTR2 a vacuolar Cu transporter and RiCTR3A a plasma membrane protein involved in Cu tolerance. RiCTR1 was more highly expressed in the extraradical mycelia (ERM) and RiCTR2 in the intraradical mycelia (IRM). In the ERM, RiCTR1 expression was up-regulated by Cu deficiency and down-regulated by Cu toxicity. RiCTR2 expression increased only in the ERM grown under severe Cu-deficient conditions. These data suggest that RiCTR1 is involved in Cu uptake by the ERM and RiCTR2 in mobilization of vacuolar Cu stores. Cu deficiency decreased mycorrhizal colonization and arbuscule frequency, but increased RiCTR1 and RiCTR2 expression in the IRM, which suggest that the IRM has a high Cu demand. The two alternatively spliced products of RiCTR3, RiCTR3A and RiCTR3B, were more highly expressed in the ERM. Up-regulation of RiCTR3A by Cu toxicity and the yeast complementation assays suggest that RiCTR3A might function as a Cu receptor involved in Cu tolerance.

Keywords

arbuscular mycorrhizal fungi; copper homeostasis; copper transporters; CTR family; Rhizophagus irregularis; symbiosis

Authors

GOMEZ-GALLEGO, T.; BENABDELLAH, K.; MERLOS RODRIGO, M.; JIMENEZ JIMENEZ, A.; ALCON, C.; BERTHOMIEU, P.; FERROL, N.

Released

16. 5. 2019

ISBN

1664-462X

Periodical

Frontiers in Plant Science

Year of study

10

Number

604

State

Swiss Confederation

Pages from

1

Pages to

16

Pages count

16

URL

https://www.frontiersin.org/articles/10.3389/fpls.2019.00604/full

BibTex

@article{BUT163402,
  author="GOMEZ-GALLEGO, T. and BENABDELLAH, K. and MERLOS RODRIGO, M. and JIMENEZ JIMENEZ, A. and ALCON, C. and BERTHOMIEU, P. and FERROL, N.",
  title="The Rhizophagus irregularis Genome Encodes Two CTR Copper Transporters That Mediate Cu Import Into the Cytosol and a CTR-Like Protein Likely Involved in Copper Tolerance
",
  journal="Frontiers in Plant Science",
  year="2019",
  volume="10",
  number="604",
  pages="1--16",
  doi="10.3389/fpls.2019.00604",
  issn="1664-462X",
  url="https://www.frontiersin.org/articles/10.3389/fpls.2019.00604/full"
}