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BERNATOVA, S. SAMEK, O. OBRUČA, S. SERY, M. ZEMANEK, P. MÁROVÁ, I.
Original Title
Time evolution of trapped single cell microorganism
Type
conference paper
Language
English
Original Abstract
The combination of optical tweezers and Raman micro-spectroscopy is frequently referred as Raman tweezers. A single focused laser beam is utilized here both as a source of Raman scattering and a source forming an optical trap. Raman tweezers have been recently used in variety of applications in cell biology as a useful tool for non-contact and non-destructive determination of living cells properties. Here we use Raman tweezers to follow response of cells on the length of their cultivation in mineral oil. Analyses of obtained Raman spectra are based on 2D correlation analysis and allow us to determine the chemical background of the cell response in a gentle way.
Keywords
Raman tweezers; Raman spectroscopy; bacteria; polyhydroxyalkanoates
Authors
BERNATOVA, S.; SAMEK, O.; OBRUČA, S.; SERY, M.; ZEMANEK, P.; MÁROVÁ, I.
Released
1. 2. 2016
Publisher
SPIE
Location
San Francisco
ISBN
978-1-62841-945-0
Book
IMAGING, MANIPULATION, AND ANALYSIS OF BIOMOLECULES, CELLS, AND TISSUES IX
Pages from
1
Pages to
5
Pages count
URL
https://www.spiedigitallibrary.org/conference-proceedings-of-spie/9711/1/Time-evolution-of-trapped-single-cell-microorganism/10.1117/12.2213264.short?SSO=1
BibTex
@inproceedings{BUT163455, author="BERNATOVA, S. and SAMEK, O. and OBRUČA, S. and SERY, M. and ZEMANEK, P. and MÁROVÁ, I.", title="Time evolution of trapped single cell microorganism", booktitle="IMAGING, MANIPULATION, AND ANALYSIS OF BIOMOLECULES, CELLS, AND TISSUES IX", year="2016", pages="1--5", publisher="SPIE", address="San Francisco", doi="10.1117/12.2213264", isbn="978-1-62841-945-0", url="https://www.spiedigitallibrary.org/conference-proceedings-of-spie/9711/1/Time-evolution-of-trapped-single-cell-microorganism/10.1117/12.2213264.short?SSO=1" }