Publication detail

Characterisation of the yeast and mould biota in traditional white pickled cheeses by culture-dependent and independent molecular techniques

ŠURANSKÁ, H. RASPOR, P. UROIC, K. GOLIC, N. KOS, B. MIHAJLOVIC, S.BEGOVIC, J. SUSKOVIC, J. TOPISIROVIC, L. CADEZ, N.

Original Title

Characterisation of the yeast and mould biota in traditional white pickled cheeses by culture-dependent and independent molecular techniques

Type

journal article in Web of Science

Language

English

Original Abstract

Artisanal white pickled cheese of Western Serbia is a product of complex microbial community which detection by culture-dependent method only is hampered by its limitations. Thus, in the present study, we used a culture-independent, semi-quantitative technique based on construction of an internal transcribed spacer (ITS)-clone library from metagenomic DNA. This approach, based on direct DNA extraction followed by amplification of fungal internal transcribed regions (ITS) cloned into plasmid and restricted by endonucleases, revealed greater species richness in analysed cheeses and their by-products (17 species in total) compared to the more commonly used techniques of the culture-dependent method (8 species) and LSU-DGGE (10 species). The most frequently occurring yeast species which are commonly associated with cheeses production were Debaryomyces hansenii, Kluyveromyces lactis and Candida zeylanoides. On the other hand, Yarrowia lipolytica and Galactomyces geotrichum were detected only in one cheese sample. Moreover, some species, mainly moulds (Filobasidium globisporum, Cladosporium sp., Aspergillus sp. or Alternaria sp.) were identified only by culture-independent methods. The discrepancies between the techniques were confirmed by low correlation factor and by different indices of general biodiversity and dominance of species. The ITS-clone library approach provides the opportunity to analyse complex fungal communities associated with food products.

Keywords

DNA; Fungal DNA; ITS Ribosomal Spacer; cheese; identification

Authors

ŠURANSKÁ, H.; RASPOR, P.; UROIC, K.; GOLIC, N.; KOS, B.; MIHAJLOVIC, S.;BEGOVIC, J.; SUSKOVIC, J.; TOPISIROVIC, L.; CADEZ, N.

Released

1. 11. 2016

Publisher

Springer

ISBN

0015-5632

Periodical

Folia Microbiol

Year of study

61

Number

6

State

Czech Republic

Pages from

455

Pages to

463

Pages count

9

URL

https://link.springer.com/article/10.1007%2Fs12223-016-0455-x

BibTex

@article{BUT163460,
  author="ŠURANSKÁ, H. and RASPOR, P. and UROIC, K. and GOLIC, N. and KOS, B. and MIHAJLOVIC, S. and BEGOVIC, J. and SUSKOVIC, J. and TOPISIROVIC, L. and CADEZ, N.",
  title="Characterisation of the yeast and mould biota in traditional white pickled cheeses by culture-dependent and independent molecular techniques",
  journal="Folia Microbiol",
  year="2016",
  volume="61",
  number="6",
  pages="455--463",
  doi="10.1007/s12223-016-0455-x",
  issn="0015-5632",
  url="https://link.springer.com/article/10.1007%2Fs12223-016-0455-x"
}