Publication detail

Characterization of a long-chain alpha-galactosidase from Papiliotrema flavescens

STRATILOVA, B. KLAUDINY, J. REHULKA, P. STRATILOVA, E. MESZAROSOVA, C. GARAJOVA, S. PAVLATOVSKÁ, B. REHULKOVA, H. KOZMON, S. SESTAK, S. FIRAKOVA, Z. VADKERTIOVA, R.

Original Title

Characterization of a long-chain alpha-galactosidase from Papiliotrema flavescens

Type

journal article in Web of Science

Language

English

Original Abstract

alpha-Galactosidases are assigned to the class of hydrolases and the subclass of glycoside hydrolases (GHs). They belong to six GH families and include the only characterized alpha-galactosidases from yeasts (GH 27, Saccharomyces cerevisiae). The present study focuses on an investigation of the lactose-inducible alpha-galactosidase produced by Papiliotrema flavescens. The enzyme was present on the surface of cells and in the cytosol. Its temperature optimum was about 60 degrees C and the pH optimum was 4.8; the pH stability ranged from 3.2 to 6.6. This alpha-galactosidase also exhibited transglycosylation activity. The cytosol alpha-galactosidase with a molecular weight about 110 kDa, was purified using a combination of liquid chromatography techniques. Three intramolecular peptides were determined by the partial structural analysis of the sequences of the protein isolated, using MALDI-TOF/TOF mass spectrometry. The data obtained recognized the first yeast alpha-galactosidase, which belongs to the GH 36 family. The bioinformatics analysis and homology modeling of a 210 amino acids long C-terminal sequence (derived from cDNA) confirmed the correctness of these findings. The study was also supplemented by the screening of capsular cryptococcal yeasts, which produce the surface lactose-inducible alpha- and beta-galactosidases. The production of the lactose-inducible alpha-galactosidases was not found to be a general feature within the yeast strains examined and, therefore, the existing hypothesis on the general function of this enzyme in cryptococcal capsule rearrangement cannot be confirmed.

Keywords

alpha-Galactosidase; Cryptococcus flavescens; GH 36 family; Lactose-inducible; Long-chain; Papiliotrema flavescens

Authors

STRATILOVA, B.; KLAUDINY, J.; REHULKA, P.; STRATILOVA, E.; MESZAROSOVA, C.; GARAJOVA, S.; PAVLATOVSKÁ, B.; REHULKOVA, H.; KOZMON, S.; SESTAK, S.; FIRAKOVA, Z.; VADKERTIOVA, R.

Released

1. 2. 2018

Publisher

Springer

ISBN

0959-3993

Periodical

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

Year of study

34

Number

2

State

Kingdom of the Netherlands

Pages from

1

Pages to

14

Pages count

14

URL

https://link.springer.com/article/10.1007/s11274-017-2403-6

BibTex

@article{BUT163474,
  author="STRATILOVA, B. and KLAUDINY, J. and REHULKA, P. and STRATILOVA, E. and MESZAROSOVA, C. and GARAJOVA, S. and PAVLATOVSKÁ, B. and REHULKOVA, H. and KOZMON, S. and SESTAK, S. and FIRAKOVA, Z. and VADKERTIOVA, R.",
  title="Characterization of a long-chain alpha-galactosidase from Papiliotrema flavescens",
  journal="WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY",
  year="2018",
  volume="34",
  number="2",
  pages="1--14",
  doi="10.1007/s11274-017-2403-6",
  issn="0959-3993",
  url="https://link.springer.com/article/10.1007/s11274-017-2403-6"
}