Publication detail

Quantitative Phase Dynamics of Cancer Cell Populations Affected by Blue Light

FEITH, M. VIČAR, T. GUMULEC, J. RAUDENSKÁ, M. GJÖRLOFF WINGREN, A. MASAŘÍK, M. BALVAN, J.

Original Title

Quantitative Phase Dynamics of Cancer Cell Populations Affected by Blue Light

Type

journal article in Web of Science

Language

English

Original Abstract

Increased exposition to blue light may induce many changes in cell behavior and significantly affect the critical characteristics of cells. Here we show that multimodal holographic microscopy (MHM) within advanced image analysis is capable of correctly distinguishing between changes in cell motility, cell dry mass, cell density, and cell death induced by blue light. We focused on the effect of blue light with a wavelength of 485 nm on morphological and dynamical parameters of four cell lines, malignant PC-3, A2780, G361 cell lines, and the benign PNT1A cell line. We used MHM with blue light doses 24 mJ/cm2, 208 mJ/cm2 and two kinds of expositions (500 and 1000 ms) to acquire real-time quantitative phase information about cellular parameters. It has been shown that specific doses of the blue light significantly influence cell motility, cell dry mass and cell density. These changes were often specific for the malignant status of tested cells. Blue light dose 208 mJ/cm2 × 1000 ms affected malignant cell motility but did not change the motility of benign cell line PNT1A. This light dose also significantly decreased proliferation activity in all tested cell lines but was not so deleterious for benign cell line PNT1A as for malignant cells. Light dose 208 mJ/cm2 × 1000 ms oppositely affected cell mass in A2780 and PC-3 cells and induced different types of cell death in A2780 and G361 cell lines. Cells obtained the least damage on lower doses of light with shorter time of exposition.

Keywords

holographic microscopy; quantitative phase imaging; blue light; cell mass; cell motility; cell death

Authors

FEITH, M.; VIČAR, T.; GUMULEC, J.; RAUDENSKÁ, M.; GJÖRLOFF WINGREN, A.; MASAŘÍK, M.; BALVAN, J.

Released

9. 4. 2020

Publisher

MDPI

Location

4052 Basel, Switzerland

ISBN

2076-3417

Periodical

Applied Sciences - Basel

Year of study

10

Number

7

State

Swiss Confederation

Pages from

1

Pages to

13

Pages count

13

URL

https://www.mdpi.com/2076-3417/10/7/2597

Full text in the Digital Library

http://hdl.handle.net/11012/189003

BibTex

@article{BUT163786,
  author="Marek {Feith} and Tomáš {Vičar} and Jaromír {Gumulec} and Martina {Raudenská} and Anette {Gjörloff Wingren} and Michal {Masařík} and Jan {Balvan}",
  title="Quantitative Phase Dynamics of Cancer Cell Populations Affected by Blue Light",
  journal="Applied Sciences - Basel",
  year="2020",
  volume="10",
  number="7",
  pages="1--13",
  doi="10.3390/app10072597",
  issn="2076-3417",
  url="https://www.mdpi.com/2076-3417/10/7/2597"
}