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ADÁMKOVÁ, K. HRUBANOVÁ, K. SAMEK, O. TRUDIČOVÁ, M. SEDLÁČEK, P. KRZYŽÁNEK, V.
Original Title
Structure investigation of hydrogels using a cryo-SEM
Type
conference paper
Language
English
Original Abstract
The aim of this study is to examine and describe the ultrastructure of a selected hydrogel, namely hyaluronic acid and agarose. Several types of low temperature sample preparation protocols were compared. Especially, the effect of various freeze-etching times was investigated. In addition, besides the ultrastructural characterization by cryo-SEM the chemical analysis of selected samples was performed by Raman spectroscopy (RS). RS is an optical method enabling chemical analysis of a microscopic sample by laser beam. Fast and reliable monitoring and identification of chemical additives specific for selected hydrogels is performed by a library of spectra in the so-called fingerprint area. Experimental results can contribute to the widening of knowledge about the influence of hydrogel composition on its structure. The comparison of selected techniques can be recognized as suitable for the structural and chemical analysis applicable to other hydrogel systems as well.
Keywords
hydrogel, cryo-SEM, agarose, hyaluronic acid
Authors
ADÁMKOVÁ, K.; HRUBANOVÁ, K.; SAMEK, O.; TRUDIČOVÁ, M.; SEDLÁČEK, P.; KRZYŽÁNEK, V.
Released
1. 1. 2018
Publisher
ACAD SCIENCES, CZECH REPUBLIC, INST SCIENTIFIC INSTRUMENTS ASCR, V V I, KRALOVOPOLSKA 147, BRNO, 612 64, CZECH REPUBLIC
Location
Brno, CZECH REPUBLIC
ISBN
978-80-87441-23-7
Book
16th International Seminar of the Institute-of-Scientific-Instruments-The-Czech-Academy-of-Sciences
Edition
1
Edition number
Pages from
26
Pages to
28
Pages count
3
BibTex
@inproceedings{BUT163790, author="ADÁMKOVÁ, K. and HRUBANOVÁ, K. and SAMEK, O. and TRUDIČOVÁ, M. and SEDLÁČEK, P. and KRZYŽÁNEK, V.", title="Structure investigation of hydrogels using a cryo-SEM", booktitle="16th International Seminar of the Institute-of-Scientific-Instruments-The-Czech-Academy-of-Sciences", year="2018", series="1", number="1", pages="26--28", publisher="ACAD SCIENCES, CZECH REPUBLIC, INST SCIENTIFIC INSTRUMENTS ASCR, V V I, KRALOVOPOLSKA 147, BRNO, 612 64, CZECH REPUBLIC", address="Brno, CZECH REPUBLIC", isbn="978-80-87441-23-7" }