Publication detail

Lycorine and UV-C stimulate phenolic secondary metabolites production and miRNA expression in Chlamydomonas reinhardtii

KOLÁČKOVÁ, M. CHALOUPSKÝ, P. CERNEI, N. KLEJDUS, B. HÚSKA, D. ADAM, V.

Original Title

Lycorine and UV-C stimulate phenolic secondary metabolites production and miRNA expression in Chlamydomonas reinhardtii

Type

journal article in Web of Science

Language

English

Original Abstract

Studying stress pathways on the level of secondary metabolites that are found in very small concentration in the cells is complicated. In the algae, the role of individual metabolites (such as carotenoids, phenolic compounds, organic acids, and vitamins) and miRNAs that participate in plant's defence are very poorly understood during stressful conditions. Therefore, in the present experiment, the model organism Chlamydomonas reinhardtii was exposed to stress conditions (Lyc and UV-C irradiation) to detect these substances, even at very low concentrations. The purpose was to monitored changes at each response level with a future view to identifying their specific roles under different stress factors. In stress-treated cultures, numerous transcriptomic and metabolomic pathways were triggered in C. reinhardtii. Although Lyc significantly decreased the concentration of AA, suggesting that Lyc has a similar function in C. reinhardtii as in plants. The negative effect of UV-C radiation was based on the production of ROS and enhancement of antioxidant responses, resulting in increased levels of polyphenols and simple phenolic compounds. Both treatments did lead to extensive changes in transcript levels and miRNA expression patterns.

Keywords

Lyc; UV-C irradiation; Chlamydomonas reinhardtii; miRNA; Secondary metabolites; Stress genes

Authors

KOLÁČKOVÁ, M.; CHALOUPSKÝ, P.; CERNEI, N.; KLEJDUS, B.; HÚSKA, D.; ADAM, V.

Released

5. 6. 2020

ISBN

0304-3894

Periodical

Journal of Hazardous Materials

Year of study

391

Number

122088

State

Kingdom of the Netherlands

Pages from

1

Pages to

10

Pages count

10

URL

https://doi.org/10.1016/j.jhazmat.2020.122088

BibTex

@article{BUT163926,
  author="Martina {Koláčková} and Pavel {Chaloupský} and Natalia Vladimirovna {Cernei} and Bořivoj {Klejdus} and Dalibor {Húska} and Vojtěch {Adam}",
  title="Lycorine and UV-C stimulate phenolic secondary metabolites production and miRNA expression in Chlamydomonas reinhardtii",
  journal="Journal of Hazardous Materials",
  year="2020",
  volume="391",
  number="122088",
  pages="1--10",
  doi="10.1016/j.jhazmat.2020.122088",
  issn="0304-3894",
  url="https://doi.org/10.1016/j.jhazmat.2020.122088"
}