Publication detail

Peptide-carbon quantum dots conjugate, derived from human Retinoic Acid Receptor Responder Protein 2, against antibiotic-resistant gram positive and gram negative pathogenic bacteria

MAZUMDAR, A. HADDAD, Y. MILOSAVLJEVIĆ, V. MICHÁLKOVÁ, H. GURÁŇ, R. BHOWMICK, S. MOULICK, A.

Original Title

Peptide-carbon quantum dots conjugate, derived from human Retinoic Acid Receptor Responder Protein 2, against antibiotic-resistant gram positive and gram negative pathogenic bacteria

Type

journal article in Web of Science

Language

English

Original Abstract

Antibiotic-resistant bacterial infections have become global issues for public health, which increases the utter need to develop alternatives to antibiotics. Here, the HSER (Homo sapiens retinoic acid receptor) peptide was designed from retinoic acid receptor responder protein 2 of Homo sapiens, and was conjugated with synthesized CQDs (carbon quantum dots) for enhanced antibacterial activity in combination, as individually they are not highly effective. The HSER-CQDs were characterized using spectrophotometer, HPLC coupled with electrospray-ionization quadrupole time-of-flight mass spectrometer (ESI-qTOF) mass spectrometer, zeta potential, zeta size, and FTIR. Thereafter, the antibacterial activity against Vancomycin-Resistant Staphylococcus aureus (VRSA) and Escherichia coli (carbapenem resistant) was studied using growth curve analysis, further supported by microscopic images showing the presence of cell debris and dead bacterial cells. The antibacterial mechanism of HSER-CQDs was observed to be via cell wall disruption and also interaction with gDNA (genomic DNA). Finally, toxicity test against normal human epithelial cells showed no toxicity, confirmed by microscopic analysis. Thus, the HSER-CQDs conjugate, having high stability and low toxicity with prominent antibacterial activity, can be used as a potential antibacterial agent.

Keywords

bacterial infections; antibiotic-resistant; carbon quantumdots; antibacterial activity; toxicity

Authors

MAZUMDAR, A.; HADDAD, Y.; MILOSAVLJEVIĆ, V.; MICHÁLKOVÁ, H.; GURÁŇ, R.; BHOWMICK, S.; MOULICK, A.

Released

28. 2. 2020

Publisher

MDPI

ISBN

2079-4991

Periodical

Nanomaterials

Year of study

10

Number

1

State

Swiss Confederation

Pages from

1

Pages to

19

Pages count

19

URL

https://www.mdpi.com/2079-4991/10/2/325

Full text in the Digital Library

http://hdl.handle.net/11012/188105

BibTex

@article{BUT164052,
  author="Aninda {Mazumdar} and Yazan Abdulmajeed Eyadh {Haddad} and Vedran {Milosavljević} and Hana {Michálková} and Roman {Guráň} and Sukanya {Bhowmick} and Amitava {Moulick}",
  title="Peptide-carbon quantum dots conjugate, derived from human Retinoic Acid Receptor Responder Protein 2, against antibiotic-resistant gram positive and gram negative pathogenic bacteria",
  journal="Nanomaterials",
  year="2020",
  volume="10",
  number="1",
  pages="1--19",
  doi="10.3390/nano10020325",
  issn="2079-4991",
  url="https://www.mdpi.com/2079-4991/10/2/325"
}