Publication detail

Inkjet-printed electrochemically reduced graphene oxide microelectrode as a platform for HT-2 mycotoxin immunoenzymatic biosensing

KUDR, J. ZHAO, L. NGUYEN, E.P. AROLA, H. NEVANEN, T.K. ADAM, V. ZÍTKA, O. MERKOCI, A.

Original Title

Inkjet-printed electrochemically reduced graphene oxide microelectrode as a platform for HT-2 mycotoxin immunoenzymatic biosensing

Type

journal article in Web of Science

Language

English

Original Abstract

The design and application of an inkjet-printed electrochemically reduced graphene oxide microelectrode for HT-2 mycotoxin immunoenzymatic biosensing is reported. A water-based graphene oxide ink was first formulated and single-drop line working microelectrodes were inkjet-printed onto poly(ethylene 2,6-naphthalate) substrates, with dimensions of 78 mu m in width and 30 nm in height after solvent evaporation. The printed graphene oxide microelectmdes were electrochemically reduced and characterized by Raman and X-ray photoelectron spectroscopies in addition to microscopies. Through optimization of the electrochemical reduction parameters, differential pulse voltammetry were performed to examine the sensing of 1-naphthol (1-N), where it was revealed that reduction times had significant effects on electrode performance. The developed microelectrodes were then used as an immunoenzymatic biosensor for the detection of HT-2 mycotoxin based on carbodiimide linking of the microelectmde surface and HT-2 toxin antigen binding fragment of antibody (anti-HT2 (10) Fab). The HT-2 toxin and anti-HT2 (10) Fab reaction was reported by anti-HT2 immune complex single-chain variable fragment of antibody fused with alkaline phosphatase (anti-IC-HT2 scFv-ALP) which is able to produce an electmactive reporter - 1-N. The biosensor showed detection limit of 1.6 ng . mL(-1) and a linear dynamic range of 6.3 - 100.0 ng . mL(-1) within a 5 min incubation with 1-naphthyl phosphate (1-NP) substrate.

Keywords

2D material; Antibody; Biosensor; Electrochemistry; Graphene oxide

Authors

KUDR, J.; ZHAO, L.; NGUYEN, E.P.; AROLA, H.; NEVANEN, T.K.; ADAM, V.; ZÍTKA, O.; MERKOCI, A.

Released

15. 5. 2020

ISBN

0956-5663

Periodical

BIOSENSORS & BIOELECTRONICS

Year of study

156

Number

112109

State

Kingdom of the Netherlands

Pages from

1

Pages to

8

Pages count

8

URL

https://doi.org/10.1016/j.bios.2020.112109

BibTex

@article{BUT164097,
  author="KUDR, J. and ZHAO, L. and NGUYEN, E.P. and AROLA, H. and NEVANEN, T.K. and ADAM, V. and ZÍTKA, O. and MERKOCI, A.",
  title="Inkjet-printed electrochemically reduced graphene oxide microelectrode as a platform for HT-2 mycotoxin immunoenzymatic biosensing",
  journal="BIOSENSORS & BIOELECTRONICS",
  year="2020",
  volume="156",
  number="112109",
  pages="1--8",
  doi="10.1016/j.bios.2020.112109",
  issn="0956-5663",
  url="https://doi.org/10.1016/j.bios.2020.112109"
}