Publication detail

Electrochemical biosensor based on modified reduced graphene oxide with silver nanoparticles for detection of methylated DNA

SEDLÁČKOVÁ, E. BIRGUSOVÁ, E. BYTEŠNÍKOVÁ, Z. ŠVEC, P. RICHTERA, L. ADAM, V.

Original Title

Electrochemical biosensor based on modified reduced graphene oxide with silver nanoparticles for detection of methylated DNA

Type

conference paper

Language

English

Original Abstract

DNA methylation is one of the most studied and basic epigenetic process related to several diseases such as diabetes, neurodegenerative or cardiovascular diseases and even cancer. Methylated DNA presents a new generation of biomarkers which can be used for point-of-care detection. Electrochemical biosensors provide simple, fast, cost-effective, easy-to-use, reliable and efficient detection in contrast with conventional diagnostic methods. These biosensors can be used for early diagnosis of mentioned diseases and increase patient recovery by early clinical interventions. In this study, methylated DNA was detected electrochemically by the developed biosensor. The bare gold electrode was modified by drop-casting reduced graphene oxide with silver nanoparticles which enhance electrochemical signal due to their strong affinity to thiol modified DNA probe via a disulfide bridge. Afterwards, the sensitivity and selectivity of the nanocomposite were examined. The faradic electrochemical impedance spectroscopy was used for determination of the hybridization of the DNA probe with a methylated DNA sequence. The fabricated biosensor shows promising analytical features with a wide detection of the linear range.

Keywords

Reduced graphene oxide; silver nanoparticles; nanomaterials; methylated DNA; electrochemical biosensor

Authors

SEDLÁČKOVÁ, E.; BIRGUSOVÁ, E.; BYTEŠNÍKOVÁ, Z.; ŠVEC, P.; RICHTERA, L.; ADAM, V.

Released

10. 6. 2020

Publisher

Tanger

ISBN

978-80-87294-95-6

Book

NANOCON 2019 CONFERENCE PROCEEDINGS

Edition

1.

Pages from

391

Pages to

395

Pages count

5

URL

https://doi.org/10.37904/nanocon.2019.8610

Full text in the Digital Library

http://hdl.handle.net/11012/196668

BibTex

@inproceedings{BUT164255,
  author="Eliška {Dostálová} and Eliška {Birgusová} and Zuzana {Bytešníková} and Pavel {Švec} and Lukáš {Richtera} and Vojtěch {Adam}",
  title="Electrochemical biosensor based on modified reduced graphene oxide with silver nanoparticles for detection of methylated DNA",
  booktitle="NANOCON 2019 CONFERENCE PROCEEDINGS",
  year="2020",
  series="1.",
  pages="391--395",
  publisher="Tanger",
  doi="10.37904/nanocon.2019.8610",
  isbn="978-80-87294-95-6",
  url="https://doi.org/10.37904/nanocon.2019.8610"
}