Publication detail

Nanoparticle-based FRET pair selection for investigation of metallothionein dimerization

PAVELICOVÁ, K. POMPEIANO VANÍČKOVÁ, L. VLČNOVSKÁ, M. VACULOVIČOVÁ, M.

Original Title

Nanoparticle-based FRET pair selection for investigation of metallothionein dimerization

Type

conference paper

Language

English

Original Abstract

Metalloproteins (MTs) are one of the most diverse classes of proteins, with the intrinsic metal atoms providing a catalytic, regulatory and structural role crucial to protein functioning. When stored under aerobic conditions, MTs might form dimers (as well as higher oligomers) that play an essential role as mediators in oxidoreduction signalling pathways. In this work, we explore the non-oxidative dimerization process characterized by metal (Cd) bridging of MT monomers by Förster resonance energy transfer. The formation of MT dimers/oligomers was investigated by applying capillary electrophoresis and matrix-assisted laser desorption/ionization with mass spectrometric detection.

Keywords

Metallothionein; dimerization; capillary electrophoresis; Förster resonance energy transfer

Authors

PAVELICOVÁ, K.; POMPEIANO VANÍČKOVÁ, L.; VLČNOVSKÁ, M.; VACULOVIČOVÁ, M.

Released

10. 6. 2020

Publisher

Tanger

ISBN

978-80-87294-95-6

Book

NANOCON 2019 CONFERENCE PROCEEDINGS

Edition

1.

Pages from

406

Pages to

410

Pages count

5

URL

https://doi.org/10.37904/nanocon.2019.8566

Full text in the Digital Library

http://hdl.handle.net/11012/196669

BibTex

@inproceedings{BUT164256,
  author="Kristýna {Pavelicová} and Lucie {Pompeiano Vaníčková} and Marcela {Vlčnovská} and Markéta {Vaculovičová}",
  title="Nanoparticle-based FRET pair selection for investigation of metallothionein dimerization",
  booktitle="NANOCON 2019 CONFERENCE PROCEEDINGS",
  year="2020",
  series="1.",
  pages="406--410",
  publisher="Tanger",
  doi="10.37904/nanocon.2019.8566",
  isbn="978-80-87294-95-6",
  url="https://doi.org/10.37904/nanocon.2019.8566"
}