Publication detail

Determination of trolox equivalent antioxidant capacity in berries using amperometric tyrosinase biosensor based on multi-walled carbon nanotubes

FRANGU, A. ASHRAFI, A. SÝS, M. ARBNESHI, T. METELKA, R. ADAM, V. VLČEK, M. RICHTERA, L.

Original Title

Determination of trolox equivalent antioxidant capacity in berries using amperometric tyrosinase biosensor based on multi-walled carbon nanotubes

Type

journal article in Web of Science

Language

English

Original Abstract

In this contribution, Trolox equivalent antioxidant capacity (TEAC) was determined in various berries using carbon paste tyrosinase biosensor with multi-walled carbon nanotubes (MWCNTs), coated with Nafion((R)) layer. Electrochemical behaviour of the biosensor and influence of MWCNTs on carbon paste surface were studied with respect to the sensitive amperometric detection of total content of phenolic compounds in berries, expressed as concentration equivalent of Trolox. After optimization of key instrumental and electroanalytical parameters, the biosensor was used for determination of TEAC in blackberries, blueberries, cranberries, raspberries and strawberries by method of multiple standard additions. Electrochemical TEAC assays corresponded well with results obtained by spectrophotometric 1,1-diphenyl-2-picrylhydrazyl radical method, known as DPPH assay. Obtained values were compared with those listed in the National Nutrient Database for additional antioxidant capacity assays as well.

Keywords

amperometry; ascorbic acid; berries; biosensor; multi-wall carbon nanotubes; carbon paste electrode; tyrosinase; Trolox; antioxidant capacity

Authors

FRANGU, A.; ASHRAFI, A.; SÝS, M.; ARBNESHI, T.; METELKA, R.; ADAM, V.; VLČEK, M.; RICHTERA, L.

Released

30. 4. 2020

Publisher

MDPI

ISBN

2076-3417

Periodical

Applied Sciences - Basel

Year of study

10

Number

7

State

Swiss Confederation

Pages from

1

Pages to

13

Pages count

13

URL

https://www.mdpi.com/2076-3417/10/7/2497

Full text in the Digital Library

http://hdl.handle.net/11012/194794

BibTex

@article{BUT164381,
  author="Arbër {Frangu} and Amirmansoor {Ashrafi} and Milan {Sýs} and Tahir {Arbneshi} and Radovan {Metelka} and Vojtěch {Adam} and Milan {Vlček} and Lukáš {Richtera}",
  title="Determination of trolox equivalent antioxidant capacity in berries using amperometric tyrosinase biosensor based on multi-walled carbon nanotubes",
  journal="Applied Sciences - Basel",
  year="2020",
  volume="10",
  number="7",
  pages="1--13",
  doi="10.3390/app10072497",
  issn="2076-3417",
  url="https://www.mdpi.com/2076-3417/10/7/2497"
}