Publication detail

A Simple Drug Delivery System for Platelet-Derived Bioactive Molecules, to Improve Melanocyte Stimulation in Vitiligo Treatment

VOCETKOVÁ, K. SOVKOVÁ, V. BUZGO, M. LUKÁŠOVÁ, V. DIVÍN, R. RAMPICHOVÁ, M. BLAŽEK, P. ZIKMUND, T. KAISER, J. KARPÍŠEK, Z. AMLER, E. FILOVA, E.

Original Title

A Simple Drug Delivery System for Platelet-Derived Bioactive Molecules, to Improve Melanocyte Stimulation in Vitiligo Treatment

Type

journal article in Web of Science

Language

English

Original Abstract

Vitiligo is the most common depigmentation disorder of the skin. Currently, its therapy focuses on the halting of the immune response and stimulation of the regenerative processes, leading to the restoration of normal melanocyte function. Platelet-rich plasma (PRP) represents a safe and cheap regenerative therapy option, as it delivers a wide spectrum of native growth factors, cytokines and other bioactive molecules. The aim of this study was to develop a simple delivery system to prolong the effects of the bioactive molecules released from platelets. The surface of electrospun and centrifugally spun poly-ε-caprolactone (PCL) fibrous scaffolds was functionalized with various concentrations of platelets; the influence of the morphology of the scaffolds and the concentration of the released platelet-derived bioactive molecules on melanocytes, was then assessed. An almost two-fold increase in the amount of the released bioactive molecules was detected on the centrifugally spun vs. electrospun scaffolds, and a sustained 14-day release of the bioactive molecules was demonstrated. A strong concentration-dependent response of melanocyte to the bioactive molecules was observed; higher concentrations of bioactive molecules resulted in improved metabolic activity and proliferation of melanocytes. This simple system improves melanocyte viability, offers on-site preparation and is suitable for prolonged topical PRP administration.

Keywords

vitiligo; melanocyte; platelets; electrospinning; centrifugal spinning

Authors

VOCETKOVÁ, K.; SOVKOVÁ, V.; BUZGO, M.; LUKÁŠOVÁ, V.; DIVÍN, R.; RAMPICHOVÁ, M.; BLAŽEK, P.; ZIKMUND, T.; KAISER, J.; KARPÍŠEK, Z.; AMLER, E.; FILOVA, E.

Released

10. 9. 2020

Publisher

MDPI

ISBN

2079-4991

Periodical

Nanomaterials

Year of study

10

Number

9

State

Swiss Confederation

Pages from

1

Pages to

19

Pages count

19

URL

https://www.mdpi.com/2079-4991/10/9/1801

Full text in the Digital Library

http://hdl.handle.net/11012/195709

BibTex

@article{BUT165434,
  author="Karolína {Vocetková} and Věra {Sovková} and Matěj {Buzgo} and Věra {Lukášová} and Radek {Divín} and Michaela {Rampichová} and Pavel {Blažek} and Tomáš {Zikmund} and Jozef {Kaiser} and Zdeněk {Karpíšek} and Evžen {Amler} and Eva {Filova}",
  title="A Simple Drug Delivery System for Platelet-Derived Bioactive Molecules, to Improve Melanocyte Stimulation in Vitiligo Treatment",
  journal="Nanomaterials",
  year="2020",
  volume="10",
  number="9",
  pages="1--19",
  doi="10.3390/nano10091801",
  issn="2079-4991",
  url="https://www.mdpi.com/2079-4991/10/9/1801"
}