Publication detail

A Comparative Study of Some Procedures for Isolation of Fruit DNA of Sufficient Quality for PCR-Based Assays

FIALOVÁ, L. ROMANOVSKÁ, D. MÁROVÁ, I.

Original Title

A Comparative Study of Some Procedures for Isolation of Fruit DNA of Sufficient Quality for PCR-Based Assays

Type

journal article in Web of Science

Language

English

Original Abstract

Food fraud has been and still is a problem in the food industry. It is detectable by several approaches, such as high performance liquid chromatography (HPLC), chemometric assays, or DNA-based techniques, each with its own drawbacks. This work addresses one major drawback of DNA-based methods, in particular their sensitivity to inhibitors contained in particular matrices from which DNA is isolated. We tested five commercial kits and one in-house method characterized by different ways of sample homogenization and DNA capture and purification. Using these methods, DNA was isolated from 10 different fruit species commonly used in plant-based foodstuffs. The quality of the DNA was evaluated by UV-VIS spectrophotometry. Two types of qPCR assays were used for DNA quality testing: (i) Method specific for plant ITS2 region, (ii) methods specific for individual fruit species. Based mainly on the results of real-time PCR assays, we were able to find two column-based kits and one magnetic carrier-based kit, which consistently provided fruit DNA isolates of sufficient quality for PCR-based assays useful for routine analysis and identification of individual fruit species in food products.

Keywords

DNA isolation; real-time PCR; commercial kit; red fruit; Prunus; tropical fruit

Authors

FIALOVÁ, L.; ROMANOVSKÁ, D.; MÁROVÁ, I.

Released

25. 9. 2020

Publisher

MDPI

ISBN

1420-3049

Periodical

MOLECULES

Year of study

25

Number

18

State

Swiss Confederation

Pages from

1

Pages to

15

Pages count

15

URL

https://www.mdpi.com/1420-3049/25/18/4317

Full text in the Digital Library

http://hdl.handle.net/11012/195713

BibTex

@article{BUT165467,
  author="Lenka {Fialová} and Denisa {Langová} and Ivana {Márová}",
  title="A Comparative Study of Some Procedures for Isolation of Fruit DNA of Sufficient Quality for PCR-Based Assays",
  journal="MOLECULES",
  year="2020",
  volume="25",
  number="18",
  pages="1--15",
  doi="10.3390/molecules25184317",
  issn="1420-3049",
  url="https://www.mdpi.com/1420-3049/25/18/4317"
}