Publication detail

Analysis of Bacteriophage-Host Interaction by Raman Tweezers

Zdeněk Pilát1*, Alexandr Jonáš1, Jana Pilátová2, Tereza Klementová1, Silvie Bernatová1, Martin Šiler1, Tadeáš Maňka1, Martin Kizovský1, Filip Růžička3, Roman Pantůček4, Ute Neugebauer5,6, Ota Samek1, Pavel Zemánek

Original Title

Analysis of Bacteriophage-Host Interaction by Raman Tweezers

Type

journal article in Web of Science

Language

English

Original Abstract

Bacteriophages, or "phages" for short, are viruses that replicate in bacteria. The therapeutic and biotechnological potential of phages and their lytic enzymes is of interest for their ability to selectively destroy pathogenic bacteria, including antibiotic-resistant strains. Introduction of phage preparations into medicine, biotechnology, and food industry requires a thorough characterization of phage-host interaction on a molecular level. We employed Raman tweezers to analyze the phage-host interaction of Staphylococcus aureus strain FS159 with a virulent phage JK2 (=812K1/420) of the Myoviridae family and a temperate phage 80 alpha of the Siphoviridae family. We analyzed the timeline of phage-induced molecular changes in infected host cells. We reliably detected the presence of replicating phages in bacterial cells within 5 min after infection. Our results lay the foundations for building a Raman-based diagnostic instrument capable of real-time, in vivo, in situ, nondestructive characterization of the phage-host relationship on the level of individual cells, which has the potential of importantly contributing to the development of phage therapy and enzybiotics.

Keywords

STAPHYLOCOCCUS-AUREUS; ELECTRON-TRANSPORT; VIRUS STRUCTURE; SPECTROSCOPY; PHAGE; INHIBITION; TRANSCRIPTION; EXPRESSION; CYTOCHROME; CAPSIDS

Authors

Zdeněk Pilát1*, Alexandr Jonáš1, Jana Pilátová2, Tereza Klementová1, Silvie Bernatová1, Martin Šiler1, Tadeáš Maňka1, Martin Kizovský1, Filip Růžička3, Roman Pantůček4, Ute Neugebauer5,6, Ota Samek1, Pavel Zemánek

Released

20. 8. 2020

Publisher

AMER CHEMICAL SOC

Location

WASHINGTON

ISBN

1520-6882

Periodical

Analytical Chemistry

Year of study

92

Number

18

State

United States of America

Pages from

12304

Pages to

12311

Pages count

8

URL

https://pubs.acs.org/doi/10.1021/acs.analchem.0c01963

BibTex

@article{BUT165603,
  author="Zdeněk {Pilát} and Tereza {Klementová}",
  title="Analysis of Bacteriophage-Host Interaction by Raman Tweezers",
  journal="Analytical Chemistry",
  year="2020",
  volume="92",
  number="18",
  pages="12304--12311",
  doi="10.1021/acs.analchem.0c01963",
  issn="1520-6882",
  url="https://pubs.acs.org/doi/10.1021/acs.analchem.0c01963"
}