Publication detail

PCR past, present and future

ZHU, H. ZHANG, H. XU, Y. LAŠŠÁKOVÁ, S. KORABEČNÁ, M. NEUŽIL, P.

Original Title

PCR past, present and future

Type

journal article in Web of Science

Language

English

Original Abstract

PCR has become one of the most valuable techniques currently used in bioscience, diagnostics and forensic science. Here we review the history of PCR development and the technologies that have evolved from the original PCR method. Currently, there are two main areas of PCR utilization in bioscience: high-throughput PCR systems and microfluidics-based PCR devices for point-of-care (POC) applications. We also discuss the commercialization of these techniques and conclude with a look into their modifications and use in innovative areas of biomedicine. For example, real-time reverse transcription PCR is the gold standard for SARS-CoV-2 diagnoses. It could also be used for POC applications, being a key component of the sample-to-answer system.

Keywords

COVID-19; digital PCR; microfluidics; PCR; point-of-care diagnostics; portable systems; reverse transcription PCR; RNA virus diagnoses

Authors

ZHU, H.; ZHANG, H.; XU, Y.; LAŠŠÁKOVÁ, S.; KORABEČNÁ, M.; NEUŽIL, P.

Released

20. 8. 2020

Publisher

FUTURE SCI LTD

Location

LONDON

ISBN

0736-6205

Periodical

BIOTECHNIQUES

Year of study

69

Number

4

State

United States of America

Pages from

1

Pages to

10

Pages count

10

URL

https://www.future-science.com/doi/full/10.2144/btn-2020-0057

BibTex

@article{BUT165710,
  author="ZHU, H. and ZHANG, H. and XU, Y. and LAŠŠÁKOVÁ, S. and KORABEČNÁ, M. and NEUŽIL, P.",
  title="PCR past, present and future",
  journal="BIOTECHNIQUES",
  year="2020",
  volume="69",
  number="4",
  pages="1--10",
  doi="10.2144/btn-2020-0057",
  issn="0736-6205",
  url="https://www.future-science.com/doi/full/10.2144/btn-2020-0057"
}