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SLAVÍK, J. ČMIEL, V. HUBÁLEK, J. YANG, Y. REN, T.-L.
Original Title
Hippocampal Neurons’ Alignment on Quartz Grooves and Parylene Cues on Quartz Substrate
Type
journal article in Web of Science
Language
English
Original Abstract
Alignment and patterning of neurons have great importance in some research fields, especially for regenerative medicine and for the formation of artificial neural networks. Alignment of neurons on quartz grooves and parylene cues on quartz substrate was evaluated in this work. The neurons’ alignment on quartz grooves is considered to be topographical alignment, while the neurons’ alignment on parylene cues on quartz substrate is considered to be chemical alignment. Both quartz grooves’ and parylene cues’ widths were fabricated in a range from 2 µm to 8 µm; quartz grooves’ heights were in a range from 0.25 µm to 4 µm, while parylene cues’ heights were only 0.25 µm. Neurons were dissociated hippocampal neurons from rat E18. Neurons were cultivated on test substrates for 7 days before alignment evaluation. As expected, neurons aligned according to the direction of grooves and cues; however, transversal growth direction was also observed with much smaller tendency. Chemical alignment was found to be more effective than topographical alignment. If parylene cues are thin and distanced enough, then neurons have a tendency to follow the direction of individual parylene cues; however, neurons on quartz grooves have a tendency just to follow a preferable direction than individual quartz grooves.
Keywords
neurons, quartz grooves, parylene cues, neurons alighment, neurons analysis
Authors
SLAVÍK, J.; ČMIEL, V.; HUBÁLEK, J.; YANG, Y.; REN, T.-L.
Released
30. 12. 2020
Publisher
MDPI AG
ISBN
2076-3417
Periodical
Applied Sciences - Basel
Year of study
11
Number
1
State
Swiss Confederation
Pages from
Pages to
12
Pages count
URL
https://www.mdpi.com/2076-3417/11/1/275
Full text in the Digital Library
http://hdl.handle.net/11012/195846
BibTex
@article{BUT167392, author="Jan {Slavík} and Vratislav {Čmiel} and Jaromír {Hubálek} and Yi {Yang} and Tian-Ling {Ren}", title="Hippocampal Neurons’ Alignment on Quartz Grooves and Parylene Cues on Quartz Substrate", journal="Applied Sciences - Basel", year="2020", volume="11", number="1", pages="1--12", doi="10.3390/app11010275", issn="2076-3417", url="https://www.mdpi.com/2076-3417/11/1/275" }