Publication detail

Hippocampal Neurons’ Alignment on Quartz Grooves and Parylene Cues on Quartz Substrate

SLAVÍK, J. ČMIEL, V. HUBÁLEK, J. YANG, Y. REN, T.-L.

Original Title

Hippocampal Neurons’ Alignment on Quartz Grooves and Parylene Cues on Quartz Substrate

Type

journal article in Web of Science

Language

English

Original Abstract

Alignment and patterning of neurons have great importance in some research fields, especially for regenerative medicine and for the formation of artificial neural networks. Alignment of neurons on quartz grooves and parylene cues on quartz substrate was evaluated in this work. The neurons’ alignment on quartz grooves is considered to be topographical alignment, while the neurons’ alignment on parylene cues on quartz substrate is considered to be chemical alignment. Both quartz grooves’ and parylene cues’ widths were fabricated in a range from 2 µm to 8 µm; quartz grooves’ heights were in a range from 0.25 µm to 4 µm, while parylene cues’ heights were only 0.25 µm. Neurons were dissociated hippocampal neurons from rat E18. Neurons were cultivated on test substrates for 7 days before alignment evaluation. As expected, neurons aligned according to the direction of grooves and cues; however, transversal growth direction was also observed with much smaller tendency. Chemical alignment was found to be more effective than topographical alignment. If parylene cues are thin and distanced enough, then neurons have a tendency to follow the direction of individual parylene cues; however, neurons on quartz grooves have a tendency just to follow a preferable direction than individual quartz grooves.

Keywords

neurons, quartz grooves, parylene cues, neurons alighment, neurons analysis

Authors

SLAVÍK, J.; ČMIEL, V.; HUBÁLEK, J.; YANG, Y.; REN, T.-L.

Released

30. 12. 2020

Publisher

MDPI AG

ISBN

2076-3417

Periodical

Applied Sciences - Basel

Year of study

11

Number

1

State

Swiss Confederation

Pages from

1

Pages to

12

Pages count

12

URL

Full text in the Digital Library

BibTex

@article{BUT167392,
  author="Jan {Slavík} and Vratislav {Čmiel} and Jaromír {Hubálek} and Yi {Yang} and Tian-Ling {Ren}",
  title="Hippocampal Neurons’ Alignment on Quartz Grooves and Parylene Cues on Quartz Substrate",
  journal="Applied Sciences - Basel",
  year="2020",
  volume="11",
  number="1",
  pages="1--12",
  doi="10.3390/app11010275",
  issn="2076-3417",
  url="https://www.mdpi.com/2076-3417/11/1/275"
}