Publication detail

Fundamentally different repetitive element composition of sex chromosomes in Rumex acetosa

JESIONEK, W. BODLÁKOVÁ, M. KUBÁT, Z. ČEGAN, R. VYSKOT, B. VRÁNA, J. ŠAFÁŘ, J. PUTEROVÁ, J. HOBZA, R.

Original Title

Fundamentally different repetitive element composition of sex chromosomes in Rumex acetosa

Type

journal article in Web of Science

Language

English

Original Abstract

Dioecious species with well-established sex chromosomes are rare in the plant kingdom. Most sex chromosomes increased in size but no comprehensive analysis of the kind of sequences which drive this expansion has been presented. Here, we analysed sex chromosome structure in common sorrel (Rumex acetosa), a dioecious plant with XY1Y2 sex determination, and we provide the first chromosome specific repeatome analysis for a plant species possessing sex chromosomes. We flow-sorted and separately sequenced sex chromosomes and autosomes in R. acetosa using the two-dimensional Fluorescence In Situ Hybridization in Suspension (FISHIS) method and Illumina sequencing. We identified and quantified individual repeats using RepeatExplorer, Tandem Repeat Finder and Tandem Repeats Analysis Program. We employed Fluorescence In Situ Hybridization (FISH) to analyse chromosome localisation of satellites and transposons. We identified a number of novel satellites, which have, similarly as previously known satellites, significantly expanded on the Y chromosome but not as much on the X nor on autosomes. Additionally, the size increase of Y chromosomes is caused by non-LTR and LTR retrotransposons, while only the latter ones contribute to the enlargement of the X chromosome. However, the X chromosome is populated by different LTR retrotransposon lineages than Y chromosomes. The X and Y chromosomes significantly diverged in terms of repeat composition. The destiny of the Y chromosomes has been shaped by a lack of recombination resulting in the expansion of diverse satellites and microsatellites and faster fixation of newly inserted transposable elements (TEs). In addition, X and Y chromosomes, despite similar total counts of TEs, significantly differ in representation of individual TE lineages, which indicates that transposons proliferate preferentially either in the paternal or maternal lineage.

Keywords

sex chromosomes, genome dynamics, transposable elements, satellites

Authors

JESIONEK, W.; BODLÁKOVÁ, M.; KUBÁT, Z.; ČEGAN, R.; VYSKOT, B.; VRÁNA, J.; ŠAFÁŘ, J.; PUTEROVÁ, J.; HOBZA, R.

Released

1. 1. 2021

ISBN

1095-8290

Periodical

Annals of Botany

Year of study

127

Number

1

State

United Kingdom of Great Britain and Northern Ireland

Pages from

33

Pages to

47

Pages count

15

URL

https://academic.oup.com/aob/article/127/1/33/5903355

BibTex

@article{BUT168153,
  author="JESIONEK, W. and BODLÁKOVÁ, M. and KUBÁT, Z. and ČEGAN, R. and VYSKOT, B. and VRÁNA, J. and ŠAFÁŘ, J. and PUTEROVÁ, J. and HOBZA, R.",
  title="Fundamentally different repetitive element composition of sex chromosomes in Rumex acetosa",
  journal="Annals of Botany",
  year="2021",
  volume="127",
  number="1",
  pages="33--47",
  doi="10.1093/aob/mcaa160",
  issn="1095-8290",
  url="https://academic.oup.com/aob/article/127/1/33/5903355"
}