Publication detail

Effect of load-induced local mechanical strain on peri-implant bone cell activity related to bone resorption and formation in mice: An analysis of histology and strain distributions

OKAWARA, H. ARAI, Y. MATSUNO, H. MARCIÁN, P. BORÁK, L. AOKI, K. WAKABAYASHI, N.

Original Title

Effect of load-induced local mechanical strain on peri-implant bone cell activity related to bone resorption and formation in mice: An analysis of histology and strain distributions

Type

journal article in Web of Science

Language

English

Original Abstract

The purpose of this study was to investigate the effect of load-induced local mechanical strain on bone cell activity of peri-implant bone in mice. Titanium implants were placed in the maxillae of 13-week-old male C57BL/6J mice and subjected to intermittent 0.15 N, 0.3 N, or 0.6 N loads for 30 min/day for 6 days. The animals were sacrificed 2 days after the final loading. Unloaded mice were used as controls. An animal-specific three-dimensional finite element model was constructed based on morphological data retrieved from in vivo microfocus computed tomography for each mouse to calculate the mechanical strain distribution. Strain distribution images were overlaid on corresponding histological images of the same site in the same animal. The buccal cervical region of the peri-implant bone was predetermined as the region of interest (ROI). Each ROI was divided by four strain intensity levels: 0–20 με, 20–60 με, 60–100 με, and ≥100 με, and the bone histomorphometric parameters were analyzed by the total area of each strain range for all loaded samples. The distance between the calcified front and calcein labeling as a parameter representing the mineral apposition rate was significantly greater in the areas with strain intensity ≥100 με than in the area with strain intensity <100 με, suggesting that the bone formation activity of osteoblasts was locally enhanced by a higher mechanical strain. However, the shrunken osteocytes and the empty osteocyte lacunae were significantly lower in the highest strain area, suggesting that osteoclastogenesis was more retarded in higher strain areas than in lower strain areas. The histomorphometric parameters were not affected geometrically in the unloaded animals, suggesting that the load-induced mechanical strain caused differences in the histomorphometric parameters. Our findings support the hypothesis that bone cell activity related to bone resorption and formation is local strain-dependent on implant loading.

Keywords

Dental implants; Bone remodeling; Histomorphometry; Osteoblasts; Mechanotransduction; Stress analysis

Authors

OKAWARA, H.; ARAI, Y.; MATSUNO, H.; MARCIÁN, P.; BORÁK, L.; AOKI, K.; WAKABAYASHI, N.

Released

30. 1. 2021

Publisher

Elsevier

Location

Nizozemsko

ISBN

1751-6161

Periodical

Journal of the mechanical behavior of biomedical materials

Year of study

116

Number

1

State

Kingdom of the Netherlands

Pages from

104370

Pages to

104370

Pages count

9

URL

https://www.sciencedirect.com/science/article/pii/S1751616121000618

BibTex

@article{BUT169100,
  author="Hisami {Okawara} and Yuki {Arai} and Hitomi {Matsuno} and Petr {Marcián} and Libor {Borák} and Kazuhiro {Aoki} and Noriyuki {WAKABAYASHI}",
  title="Effect of load-induced local mechanical strain on peri-implant bone cell activity related to bone resorption and formation in mice: An analysis of histology and strain distributions",
  journal="Journal of the mechanical behavior of biomedical materials",
  year="2021",
  volume="116",
  number="1",
  pages="104370--104370",
  issn="1751-6161",
  url="https://www.sciencedirect.com/science/article/pii/S1751616121000618"
}