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NOVÁČKOVÁ, I. CHATRNÁ, V. SLANINOVÁ, E. POŘÍZKA, J. SEDLÁČEK, P. OBRUČA, S.
Original Title
Evolutionary engineering approach for adaptation of PHA producing strain Halomonas halophila to levulinic and acetic acid
Type
abstract
Language
English
Original Abstract
Evolutionary engineering provides approaches useful for obtaining of microorganisms with desired characteristics on phenotype level (e.g. more effective growth, ability of utilization of different carbon sources, etc.). Characteristics of polyhydroxyalkanoates (PHAs), microbial biodegradable and biocompatible polymers, strongly depend on monomer composition. These materials could be advantageously used as an alternative of traditional petrochemical plastics. Evolved microbial strains obtained during evolutionary engineering experiments could be used for production of PHA with required properties for selected purposes. Promising halophilic PHA producer Halomonas halophila (CCM 3662) was exposed to microbial inhibitors commonly presented in hydrolysates of lignocellulosic biomass, namely acetic and levulinic acid. Multiple serial transfers of cell cultures in Erlenmeyer flasks after 48 hours were provided within adaptation experiments for more than 80 passages. Every passage has been basically characterized and selected preserved ones were also compared with wild-type strain considering potential of PHAs accumulation, effectivity of utilization of organic acids, testing of robustness and others features.
Keywords
evolutionary engineering, polyhydroxyalkanoates, Halomonas halophila, levulinic acid, acidic acid
Authors
NOVÁČKOVÁ, I.; CHATRNÁ, V.; SLANINOVÁ, E.; POŘÍZKA, J.; SEDLÁČEK, P.; OBRUČA, S.
Released
25. 2. 2021
ISBN
1314-3530
Periodical
Biotechnology and Biotechnological Equipment
Year of study
35
Number
1
State
United Kingdom of Great Britain and Northern Ireland
Pages from
25
Pages to
Pages count
BibTex
@misc{BUT169835, author="Ivana {Nováčková} and Vendula {Hrabalová} and Eva {Slaninová} and Jaromír {Pořízka} and Petr {Sedláček} and Stanislav {Obruča}", title="Evolutionary engineering approach for adaptation of PHA producing strain Halomonas halophila to levulinic and acetic acid", year="2021", journal="Biotechnology and Biotechnological Equipment", volume="35", number="1", pages="25--25", issn="1314-3530", note="abstract" }