Publication detail

A Perspective Combination Quantum Dots and Silica Nanoparticies: for Bioconjugation with Antibodies used in Immunosensors for Detection of Human Alpha-Fetoprotein

KOVAROVA, A. DRBOHLAVOVÁ, J. BÍLKOVÁ, Z. KORECKA, L.

Original Title

A Perspective Combination Quantum Dots and Silica Nanoparticies: for Bioconjugation with Antibodies used in Immunosensors for Detection of Human Alpha-Fetoprotein

Type

conference paper

Language

English

Original Abstract

The combination of quantum dots (QDs) with silica nanoparticles (SiNPs) as promising signal-generating label of antibodies used in electrochemical immunosensor is described. QDs-modified silica nanoparticles were conjugated with specific antibodies targeted to alpha-fetoprotein (AFP) to a creation of anti-AFP-SiNPs-QDs IgG conjugate for subsequent exploitation in quantum dot-linked immunosorbent assay (QLISA). Square wave anodic stripping voltammetry (SWASV) on mercury or bismuth film modified carbon screen-printed three-electrode sensors was used as a detection method. Insertion of nanoparticles to this system led to the achievement of higher current responses compare to results obtained with QDs only. Therefore, the sensitivity of complete immunoassay could be significantly improved.

Keywords

Quantum dots; Silica nanoparticles; Antibody label Electrochemical immunosensors; SWASV

Authors

KOVAROVA, A.; DRBOHLAVOVÁ, J.; BÍLKOVÁ, Z.; KORECKA, L.

Released

15. 5. 2017

ISBN

978-80-905221-5-2

Book

XXXVII MODERNI ELEKTROCHEMICKE METODY

Pages from

108

Pages to

112

Pages count

5

BibTex

@inproceedings{BUT170241,
  author="Aneta {Kovarova} and Jana {Drbohlavová} and Zuzana {Bílková} and Lucie {Korecka}",
  title="A Perspective Combination Quantum Dots and Silica Nanoparticies: for Bioconjugation with Antibodies used in Immunosensors for Detection of Human Alpha-Fetoprotein",
  booktitle="XXXVII MODERNI ELEKTROCHEMICKE METODY",
  year="2017",
  pages="108--112",
  isbn="978-80-905221-5-2"
}