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KOVAROVA, A. DRBOHLAVOVÁ, J. BÍLKOVÁ, Z. KORECKA, L.
Original Title
A Perspective Combination Quantum Dots and Silica Nanoparticies: for Bioconjugation with Antibodies used in Immunosensors for Detection of Human Alpha-Fetoprotein
Type
conference paper
Language
English
Original Abstract
The combination of quantum dots (QDs) with silica nanoparticles (SiNPs) as promising signal-generating label of antibodies used in electrochemical immunosensor is described. QDs-modified silica nanoparticles were conjugated with specific antibodies targeted to alpha-fetoprotein (AFP) to a creation of anti-AFP-SiNPs-QDs IgG conjugate for subsequent exploitation in quantum dot-linked immunosorbent assay (QLISA). Square wave anodic stripping voltammetry (SWASV) on mercury or bismuth film modified carbon screen-printed three-electrode sensors was used as a detection method. Insertion of nanoparticles to this system led to the achievement of higher current responses compare to results obtained with QDs only. Therefore, the sensitivity of complete immunoassay could be significantly improved.
Keywords
Quantum dots; Silica nanoparticles; Antibody label Electrochemical immunosensors; SWASV
Authors
KOVAROVA, A.; DRBOHLAVOVÁ, J.; BÍLKOVÁ, Z.; KORECKA, L.
Released
15. 5. 2017
ISBN
978-80-905221-5-2
Book
XXXVII MODERNI ELEKTROCHEMICKE METODY
Pages from
108
Pages to
112
Pages count
5
BibTex
@inproceedings{BUT170241, author="Aneta {Kovarova} and Jana {Drbohlavová} and Zuzana {Bílková} and Lucie {Korecka}", title="A Perspective Combination Quantum Dots and Silica Nanoparticies: for Bioconjugation with Antibodies used in Immunosensors for Detection of Human Alpha-Fetoprotein", booktitle="XXXVII MODERNI ELEKTROCHEMICKE METODY", year="2017", pages="108--112", isbn="978-80-905221-5-2" }