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Pilat, Zdenek Jezek, Jan Kizovsky, Martin Klementova, Tereza Kratky, Stanislav Sobota, Jaroslav Samek, Ota Zemanek, Pavel Buryska, Tomas Damborsky, Jiri Prokop, Zbynek
Original Title
Surface-enhanced Raman Spectroscopy in Microfluidic Chips for Directed Evolution of Enzymes and Environmental Monitoring
Type
journal article in Web of Science
Language
English
Original Abstract
Optofluidics, a research discipline combining optics with microfluidics, currently aspires to revolutionize the analysis of biological and chemical samples e.g. for medicine, pharmacology, or molecular biology. In order to detect low concentrations of analytes in water, we developed an optofluidic device containing a nanostructured substrate for surface enhanced Raman spectroscopy (SERS). The geometry of the gold surface allows localized plasmon oscillations to give rise to the SERS effect, in which the Raman spectral lines are intensified by the interaction of the plasmonic field with the electrons in the molecular bonds. The SERS substrate was enclosed in a microfluidic system, which allowed transport and precise mixing of the analyzed fluids, while preventing contamination or abrasion of the highly sensitive substrate. To illustrate its practical use, we employed the device for quantitative detection of persistent environmental pollutant 1,2,3-trichloropropane in water in millimolar and even submillimolar concentrations. The developed sensor allows fast and simple quantification of halogenated compounds and it will contribute towards the environmental monitoring and enzymology experiments with engineered haloalkane dehalogenase enzymes.
Keywords
Substrates; Springs; Raman scattering; Photonics; Electromagnetics; Measurement by laser beam; Gold
Authors
Pilat, Zdenek; Jezek, Jan; Kizovsky, Martin; Klementova, Tereza; Kratky, Stanislav; Sobota, Jaroslav; Samek, Ota; Zemanek, Pavel; Buryska, Tomas; Damborsky, Jiri; Prokop, Zbynek
Released
2. 3. 2020
Publisher
IEEE
Location
NEW YORK
ISBN
1559-9450
Book
2019 PhotonIcs & Electromagnetics Research Symposium - Spring (PIERS-Spring)
Periodical
Progress In Electromagnetics
Year of study
2019
Number
1
State
United States of America
Pages from
1301
Pages to
1309
Pages count
9
URL
https://ieeexplore-ieee-org.ezproxy.lib.vutbr.cz/document/9017521
BibTex
@article{BUT170289, author="Pilat, Zdenek and Jezek, Jan and Kizovsky, Martin and Klementova, Tereza and Kratky, Stanislav and Sobota, Jaroslav and Samek, Ota and Zemanek, Pavel and Buryska, Tomas and Damborsky, Jiri and Prokop, Zbynek", title="Surface-enhanced Raman Spectroscopy in Microfluidic Chips for Directed Evolution of Enzymes and Environmental Monitoring", journal="Progress In Electromagnetics", year="2020", volume="2019", number="1", pages="1301--1309", doi="10.1109/PIERS-Spring46901.2019.9017521", issn="1559-9450", url="https://ieeexplore-ieee-org.ezproxy.lib.vutbr.cz/document/9017521" }