Publication detail

DUCT reveals architectural mechanisms contributing to bile duct recovery in a mouse model for Alagille syndrome

HANKEOVÁ, S. ŠALPLACHTA, J. ZIKMUND, T. KAVKOVÁ, M. VAN HUL, N. BŘÍNEK, A. SMÉKALOVÁ, V. LÁZŇOVSKÝ, J. DAWIT, F. JAROŠ, J. BRYJA, V. LENDAHL, U. ELLIS, E. NEMETH, A. FISCHLER, B. HANNESO, E. KAISER, J. ANDERSSON, E.

Original Title

DUCT reveals architectural mechanisms contributing to bile duct recovery in a mouse model for Alagille syndrome

Type

journal article in Web of Science

Language

English

Original Abstract

Organ function depends on tissues adopting the correct architecture. However, insights into organ architecture are currently hampered by an absence of standardized quantitative 3D analysis. We aimed to develop a robust technology to visualize, digitalize, and segment the architecture of two tubular systems in 3D: double resin casting micro computed tomography (DUCT). As proof of principle, we applied DUCT to a mouse model for Alagille syndrome (Jag1Ndr/Ndr mice), characterized by intrahepatic bile duct paucity, that can spontaneously generate a biliary system in adulthood. DUCT identified increased central biliary branching and peripheral bile duct tortuosity as two compensatory processes occurring in distinct regions of Jag1Ndr/Ndr liver, leading to full reconstitution of wild-type biliary volume and phenotypic recovery. DUCT is thus a powerful new technology for 3D analysis, which can reveal novel phenotypes and provide a standardized method of defining liver architecture in mouse models.

Keywords

Alagille syndrome; MicroCT; cholangiopathy; human; mouse; physics of living systems; regenerative medicine; resin; stem cells; vasculature.

Authors

HANKEOVÁ, S.; ŠALPLACHTA, J.; ZIKMUND, T.; KAVKOVÁ, M.; VAN HUL, N.; BŘÍNEK, A.; SMÉKALOVÁ, V.; LÁZŇOVSKÝ, J.; DAWIT, F.; JAROŠ, J.; BRYJA, V.; LENDAHL, U.; ELLIS, E.; NEMETH, A.; FISCHLER, B.; HANNESO, E.; KAISER, J.; ANDERSSON, E.

Released

26. 2. 2021

Publisher

ELIFE SCIENCES PUBLICATIONS LTD

Location

Cambridge

ISBN

2050-084X

Periodical

eLife

Number

10

State

United Kingdom of Great Britain and Northern Ireland

Pages from

1

Pages to

29

Pages count

29

URL

https://elifesciences.org/articles/60916#content

Full text in the Digital Library

http://hdl.handle.net/11012/201001

BibTex

@article{BUT170436,
  author="Simona {Hankeová} and Jakub {Šalplachta} and Tomáš {Zikmund} and Michaela {Kavková} and Noemi {Van Hul} and Adam {Břínek} and Veronika {Smékalová} and Jakub {Lázňovský} and Feven {Dawit} and Josef {Jaroš} and Vítězslav {Bryja} and Urban {Lendahl} and ewa {ellis} and Antal {Nemeth} and Björn {Fischler} and Edouard {Hanneso} and Jozef {Kaiser} and Emma Rachel {Andersson}",
  title="DUCT reveals architectural mechanisms contributing to bile duct recovery in a mouse model for Alagille syndrome",
  journal="eLife",
  year="2021",
  number="10",
  pages="1--29",
  doi="10.7554/eLife.60916",
  issn="2050-084X",
  url="https://elifesciences.org/articles/60916#content"
}