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FOHLEROVÁ, Z. KAMNEV, K. SEPÚLVEDA SEPÚLVEDA, L. PYTLÍČEK, Z. PRÁŠEK, J. MOZALEV, A.
Original Title
Nanostructured Zirconium-Oxide Bioceramic Coatings Derived from the Anodized Al/Zr Metal Layers
Type
journal article in Web of Science
Language
English
Original Abstract
Here, zirconium-oxide ceramic coatings comprising arrays of 3D nanostructures are electrochemically synthesized, ranging in shape, size, spacing, and population density, termed as the nanomounds (o approximate to 65 nm), nanopillars (o approximate to 130 nm), and nanostumps (o approximate to 220 nm). The nanostructured coatings, alongside a flat ZrO2 anodic film, are explored as a potential biomaterial in experiments with Saos-2 cells. All coatings reveal no cytotoxicity to living cells. The population density and spreading area of the cells, being the largest on the flat film, slightly decrease with increasing nanostructure dimensions. The cells progressively proliferate on all the surfaces, the nanomounds and, especially, nanopillars promoting the best viabilities and proliferation rates. The flat, nanomound, and nanopillar coatings promote the well-defined organizations of actin filaments across the entire cell bodies with no disruption in the cytoskeletal network and the mature large dash-shaped focal adhesions. The highest activity of alkaline phosphatase and the biggest deposition of a mineralization-competent extracellular matrix occur on the nanopillar array, the other nanostructures showing a better result than the flat coating, though. The differences, paradoxes, and regularities in the cell responses are explained through the detailed consideration of the coating morphologies, surface chemistries, and processes at the cell/surface interfaces.
Keywords
anodizing; bio-interfaces; biomaterials; nanostructures; porous anodic alumina; Saos-2 cells; ZrO; (2)
Authors
FOHLEROVÁ, Z.; KAMNEV, K.; SEPÚLVEDA SEPÚLVEDA, L.; PYTLÍČEK, Z.; PRÁŠEK, J.; MOZALEV, A.
Released
25. 6. 2021
Publisher
WILEY
Location
HOBOKEN
ISBN
2196-7350
Periodical
Advanced Materials Interfaces
Number
10
State
Federal Republic of Germany
Pages from
1
Pages to
14
Pages count
URL
https://onlinelibrary.wiley.com/doi/10.1002/admi.202100256?af=R
BibTex
@article{BUT171982, author="Zdenka {Fohlerová} and Kirill {Kamnev} and Lina Marcela {Sepúlveda Sepúlveda} and Zdeněk {Pytlíček} and Jan {Prášek} and Alexander {Mozalev}", title="Nanostructured Zirconium-Oxide Bioceramic Coatings Derived from the Anodized Al/Zr Metal Layers", journal="Advanced Materials Interfaces", year="2021", number="10", pages="1--14", doi="10.1002/admi.202100256", issn="2196-7350", url="https://onlinelibrary.wiley.com/doi/10.1002/admi.202100256?af=R" }