Publication detail

Elegant, rapid and simple procedure for labeling of antibodies with fluorescent ZnCd/S quantum dots for laser ablation ICP-MS detection

ASSI, N. PAVELICOVÁ, K. VACULOVIČOVÁ, M. VACULOVIČ, T.

Original Title

Elegant, rapid and simple procedure for labeling of antibodies with fluorescent ZnCd/S quantum dots for laser ablation ICP-MS detection

Type

conference paper

Language

English

Original Abstract

Antibody labeling with a signal-providing tag (e.g. an enzyme, chromophore, fluorophore, metal chelate, radioisotope, etc.) is a key procedure in bioanalytical techniques such an enzyme-linked immunosorbent assay, western blot, or immunohistochemical analysis. The process may be laborious, impair the activity, and time-consuming. Concisely, in this work, an elegant method of rapid and effective antibody labeling by quantum dots was developed taking advantage of partial reduction of antibody structure and UV-induced quantum dot formation. Zinc and cadmium with the thiol group of the antibody as capping agents stabilizing the fluorescent nanoparticle while the biorecognition capabilities were maintained. The ZnCd/S quantum dots creation has to prosper detected with capillary electrophoresis (CE) and laser ablation ICP- MS (LA- ICP- MS).

Keywords

Antibody, antigen, quantum dots, cleavage disulfide bonds, fluorescent

Authors

ASSI, N.; PAVELICOVÁ, K.; VACULOVIČOVÁ, M.; VACULOVIČ, T.

Released

12. 7. 2021

Publisher

Tanger

ISBN

978-80-87294-98-7

Book

NANOCON 2020 CONFERENCE PROCEEDINGS

Edition

1st

ISBN

2694-930X

Periodical

Proceedings 12th International Conference on Nanomaterials - Research & Application

State

Czech Republic

Pages from

395

Pages to

398

Pages count

4

URL

https://www.confer.cz/nanocon/2020/3752-elegant-rapid-and-simple-procedure-for-labeling-of-antibodies-with-fluorescent-cdzn-s-nanoparticles-for-la-icp-ms-detection

Full text in the Digital Library

http://hdl.handle.net/11012/200499

BibTex

@inproceedings{BUT172014,
  author="Navid {Assi} and Kristýna {Pavelicová} and Markéta {Vaculovičová} and Tomáš {Vaculovič}",
  title="Elegant, rapid and simple procedure for labeling of antibodies with fluorescent ZnCd/S quantum dots for laser ablation ICP-MS detection",
  booktitle="NANOCON 2020 CONFERENCE PROCEEDINGS",
  year="2021",
  series="1st",
  journal="Proceedings 12th International Conference on Nanomaterials - Research & Application",
  pages="395--398",
  publisher="Tanger",
  doi="10.37904/nanocon.2020.3752",
  isbn="978-80-87294-98-7",
  issn="2694-930X",
  url="https://www.confer.cz/nanocon/2020/3752-elegant-rapid-and-simple-procedure-for-labeling-of-antibodies-with-fluorescent-cdzn-s-nanoparticles-for-la-icp-ms-detection"
}