Publication detail

Efficient Protein Transfection by Swarms of Chemically Powered Plasmonic Virus-Sized Nanorobots

RESSNEROVÁ, A. NOVOTNÝ, F. MICHÁLKOVÁ, H. PUMERA, M. ADAM, V. HEGER, Z.

Original Title

Efficient Protein Transfection by Swarms of Chemically Powered Plasmonic Virus-Sized Nanorobots

Type

journal article in Web of Science

Language

English

Original Abstract

Transfection is based on nonviral delivery of nucleic acids or proteins into cells. Viral approaches are being used; nevertheless, their translational capacity is nowadays decreasing due to persistent fear of their safety, therefore creating space for the field of nanotechnology. However, nanomedical approaches introducing static nanoparticles for the delivery of biologically active molecules are very likely to be overshadowed by the vast potential of nanorobotics. We hereby present a rapid nonviral transfection of protein into a difficult-to-transfect prostate cancer cell line facilitated by chemically powered rectangular virus-sized (68 nm x 33 nm) nanorobots. The enhanced diffusion of these biocompatible nanorobots is the key to their fast internalization into cells, happening in a matter of minutes and being up to 6-fold more efficient compared to static nanorobots in a nonfueled environment. The Au/Ag plasmonic nature of these nanorobots makes them simply traceable and allows for their detailed subcellular localization. Protein transfection mediated by such nanorobots is an important step forward, challenging the field of nanomedicine and having potential in future translational medical research.

Keywords

transfection; nanorobots; metallothionein; protein delivery; protein transfection

Authors

RESSNEROVÁ, A.; NOVOTNÝ, F.; MICHÁLKOVÁ, H.; PUMERA, M.; ADAM, V.; HEGER, Z.

Released

24. 8. 2021

Publisher

American Chemical Society

ISBN

1936-0851

Periodical

ACS Nano

Year of study

15

Number

8

State

United States of America

Pages from

12899

Pages to

12910

Pages count

12

URL

https://pubs.acs.org/doi/10.1021/acsnano.1c01172

Full text in the Digital Library

http://hdl.handle.net/11012/203120

BibTex

@article{BUT172541,
  author="Alžběta {Ressnerová} and Filip {Novotný} and Hana {Michálková} and Martin {Pumera} and Vojtěch {Adam} and Zbyněk {Heger}",
  title="Efficient Protein Transfection by Swarms of Chemically Powered Plasmonic Virus-Sized Nanorobots",
  journal="ACS Nano",
  year="2021",
  volume="15",
  number="8",
  pages="12899--12910",
  doi="10.1021/acsnano.1c01172",
  issn="1936-0851",
  url="https://pubs.acs.org/doi/10.1021/acsnano.1c01172"
}