Publication detail

New insights into mechanisms of copper nanoparticle toxicity in freshwater algae Chlamydomonas reinhardtii: Effects on the pathways of secondary metabolites

JÁNOVÁ, A. KOLÁČKOVÁ, M. BYTEŠNÍKOVÁ, Z. CÁPAL, P. CHALOUPSKÝ, P. ŠVEC, P. RIDOŠKOVÁ, A. CERNEI, N. KLEJDUS, B. RICHTERA, L. ADAM, V. HÚSKA, D.

Original Title

New insights into mechanisms of copper nanoparticle toxicity in freshwater algae Chlamydomonas reinhardtii: Effects on the pathways of secondary metabolites

Type

journal article in Web of Science

Language

English

Original Abstract

The effects of copper nanoparticles (Cu-NPs), including their stability in the medium, were studied with the green unicellular algae Chlamydomonas reinhardtii (CC-125). Cu-NPs were synthesized and characterized. Cu-NP particles were uniform, regular, and largely spherical, and they had smooth surfaces; the average size was estimated to be 137.4 +/- 2.1 nm. Chlamydomonas cells were cultivated for 96 h under controlled conditions in the presence of Cu-NPs, according to OECD guidelines, and then subjected to toxicological bioassays. Based on scanning electron microscopy (SEM) observations, the effects of Cu-NPs resulted in part from the dissolution of nanoparticles (NPs) and the action of copper itself, which shows the importance of studying NP stability in the testing environment. In this assay, deleterious effects were enhanced by increasing Cu-NP concentrations (5, 10, 25, 50, and 100 mg/L). Concentrations higher than 25 mg/L exhibited extreme toxicity. We confirmed the known toxic effects of metal NPs, namely, growth inhibition, reduction of chlorophyll levels in cells, cell penetration and increased ROS production. Attention was also paid to select underexplored metabolites, which were studied with a LC-MS/MS system. Treatments caused changes in metabolites profiles, and levels of p-hydroxybenzaldehyde and protocatechuic acid were especially enhanced, suggesting their positive roles in the antioxidant defence response. Furthermore, a repeatable increase in suberic acid levels was observed for various stress conditions tested, and we expect that this was the result of lipid peroxidation.

Keywords

Copper nanoparticles; Chlamydomonas; Toxicity; Penetration; Secondary metabolites; Nanoparticle stability

Authors

JÁNOVÁ, A.; KOLÁČKOVÁ, M.; BYTEŠNÍKOVÁ, Z.; CÁPAL, P.; CHALOUPSKÝ, P.; ŠVEC, P.; RIDOŠKOVÁ, A.; CERNEI, N.; KLEJDUS, B.; RICHTERA, L.; ADAM, V.; HÚSKA, D.

Released

30. 12. 2021

ISBN

2211-9264

Periodical

Algal Research

Year of study

60

Number

102476

State

Kingdom of the Netherlands

Pages from

1

Pages to

11

Pages count

11

URL

https://doi.org/10.1016/j.algal.2021.102476

BibTex

@article{BUT172676,
  author="Anna {Jánová} and Martina {Koláčková} and Zuzana {Bytešníková} and Petr {Cápal} and Pavel {Chaloupský} and Pavel {Švec} and Andrea {Ridošková} and Natalia Vladimirovna {Cernei} and Bořivoj {Klejdus} and Lukáš {Richtera} and Vojtěch {Adam} and Dalibor {Húska}",
  title="New insights into mechanisms of copper nanoparticle toxicity in freshwater algae Chlamydomonas reinhardtii: Effects on the pathways of secondary metabolites",
  journal="Algal Research",
  year="2021",
  volume="60",
  number="102476",
  pages="1--11",
  doi="10.1016/j.algal.2021.102476",
  issn="2211-9264",
  url="https://doi.org/10.1016/j.algal.2021.102476"
}