Publication detail

Flavobacterium flabelliforme sp. nov. and Flavobacterium geliluteum sp. nov., two multidrug-resistant psychrotrophic species isolated from Antarctica

KRÁLOVÁ, S. BUSSE, H. BEZDÍČEK, M. SANDOVAL-POWERS, M. NYKRÝNOVÁ, M. STAŇKOVÁ, E. KRSEK, D. SEDLÁČEK, I.

Original Title

Flavobacterium flabelliforme sp. nov. and Flavobacterium geliluteum sp. nov., two multidrug-resistant psychrotrophic species isolated from Antarctica

Type

journal article in Web of Science

Language

English

Original Abstract

Despite unfavourable Antarctic conditions, such as cold temperatures, freeze-thaw cycles, high ultraviolet radiation, dryness and lack of nutrients, microorganisms were able to adapt and surprisingly thrive in this environment. In this study, eight cold-adapted Flavobacterium strains isolated from a remote Antarctic island, James Ross Island, were studied using polyphasic taxonomic approach to determine their taxonomic position. Phylogenomic analyses based on 16S rRNA gene and 92 core genes clearly showed that these strains formed two distinct phylogenetic clusters comprising three and five strains, with average nucleotide identities significantly below 90 % in between both proposed species as well as between their closest phylogenetic relatives. Phenotyping revealed a unique pattern of biochemical and physiological characteristics enabling differentiation from the closest phylogenetically related Flavobacterium spp. Chemotaxonomic analyses showed that type strains P4023T and P7388T were characterized by the major polyamine sym-homospermidine and a quinone system containing predominantly menaquinone MK-6. In the polar lipid profile phosphatidylethanolamine, an ornithine lipid and two unidentified lipids lacking a functional group were detected as major lipids. These characteristics along with fatty acid profiles confirmed, that these species belong to the genus Flavobacterium. Thorough genomic analysis revealed presence of numerous cold-inducible or cold-adaptation associated genes, such as cold-shock proteins, proteorhodopsin, carotenoid biosynthetic genes or oxidative-stress response genes. Genomes of type strains surprisingly harboured multiple prophages, with many of them predicted to be active. Genome-mining identified biosynthetic clusters in type strain genomes with majority not matching any known biosynthetic genes which indicates further research possibilities involving these psychrotrophic bacteria. Antibiotic susceptibility testing revealed multidrug-resistant phenotype that was correlated with in silico antibiotic resistance prediction. Interestingly, while typical resistance finder tools failed to detect genes responsible for antibiotic resistance, genomic prediction confirmed multidrug-resistant profile and suggested even broader resistance than tested. Results of this study confirmed and thoroughly characterized two novel psychrotrophic Flavobacterium species, for which names Flavobacterium flabelliforme sp. nov. and Flavobacterium geliluteum sp. nov. are proposed.

Keywords

Antarctica, psychrophiles; cold-adaptation; phylogenomics; systematics; Flavobacterium flabelliforme sp. nov.; Flavobacterium geliluteum sp. nov.

Authors

KRÁLOVÁ, S.; BUSSE, H.; BEZDÍČEK, M.; SANDOVAL-POWERS, M.; NYKRÝNOVÁ, M.; STAŇKOVÁ, E.; KRSEK, D.; SEDLÁČEK, I.

Released

22. 10. 2021

Publisher

Frontiers Media SA

ISBN

1664-302X

Periodical

Frontiers in Microbiology

Year of study

12

Number

10

State

Swiss Confederation

Pages from

1

Pages to

20

Pages count

20

URL

Full text in the Digital Library

BibTex

@article{BUT172701,
  author="Stanislava {Králová} and Hans-Jürgen {Busse} and Matěj {Bezdíček} and Megan {Sandoval-Powers} and Markéta {Jakubíčková} and Eva {Staňková} and Daniel {Krsek} and Ivo {Sedláček}",
  title="Flavobacterium flabelliforme sp. nov. and Flavobacterium geliluteum sp. nov., two multidrug-resistant psychrotrophic species isolated from Antarctica",
  journal="Frontiers in Microbiology",
  year="2021",
  volume="12",
  number="10",
  pages="1--20",
  doi="10.3389/fmicb.2021.729977",
  issn="1664-302X",
  url="https://www.frontiersin.org/articles/10.3389/fmicb.2021.729977/full"
}