Publication detail

Elastin-Plasma Hybrid Hydrogels for Skin Tissue Engineering

Stojic M., Ródenas-Rochina J., López-Donaire M.L.,de Torre I.G., Pérez M.G.. ,Rodríguez-Cabello J.C., Vojtová L., Jorcano J.L., Velasco D.

Original Title

Elastin-Plasma Hybrid Hydrogels for Skin Tissue Engineering

Type

journal article in Web of Science

Language

English

Original Abstract

Dermo-epidermal equivalents based on plasma-derived fibrin hydrogels have been extensively studied for skin engineering. However, they showed rapid degradation and contraction over time and low mechanical properties which limit their reproducibility and lifespan. In order to achieve better mechanical properties, elasticity and biological properties, we incorporated a elastin-like recombinamer (ELR) network, based on two types of ELR, one modified with azide (SKS-N3) and other with cyclooctyne (SKS-Cyclo) chemical groups at molar ratio 1:1 at three different SKS (serine-lysine-serine sequence) concentrations (1, 3, and 5 wt.%), into plasma-derived fibrin hydrogels. Our results showed a decrease in gelation time and contraction, both in the absence and presence of the encapsulated human primary fibroblasts (hFBs), higher mechanical properties and increase in elasticity when SKSs content is equal or higher than 3%. However, hFBs proliferation showed an improvement when the lowest SKS content (1 wt.%) was used but started decreasing when increasing SKS concentration at day 14 with respect to the plasma control. Proliferation of human primary keratinocytes (hKCs) seeded on top of the hybrid-plasma hydrogels containing 1 and 3% of SKS showed no differences to plasma control and an increase in hKCs proliferation was observed for hybrid-plasma hydrogels containing 5 wt.% of SKS. These promising results showed the need to achieve a balance between the reduced contraction, the better mechanical properties and biological properties and indicate the potential of using this type of hydrogel as a testing platform for pharmaceutical products and cosmetics, and future work will elucidate their potential.

Keywords

bilayered in vitro skin substitutes, human plasma-derived fibrin hydrogels, fibrin hydrogels, hybrid plasma-elastin hydrogels, skin tissue engineering

Authors

Stojic M., Ródenas-Rochina J., López-Donaire M.L.,de Torre I.G., Pérez M.G.. ,Rodríguez-Cabello J.C., Vojtová L., Jorcano J.L., Velasco D.

Released

28. 6. 2021

ISBN

2073-4360

Periodical

Polymers

Year of study

13

Number

13

State

Swiss Confederation

Pages from

1

Pages to

23

Pages count

23

URL

https://www.mdpi.com/2073-4360/13/13/2114

BibTex

@article{BUT172848,
  author="Lucy {Vojtová} and Marija {Stojic}",
  title="Elastin-Plasma Hybrid Hydrogels for Skin Tissue Engineering",
  journal="Polymers",
  year="2021",
  volume="13",
  number="13",
  pages="1--23",
  doi="10.3390/polym13132114",
  issn="2073-4360",
  url="https://www.mdpi.com/2073-4360/13/13/2114"
}